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The role of IL-1β and TNF-α in hyper-reactivity of neutrophils in rapidly progressive periodontitis patients
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNA OF STOMTAOLOGY
- Issue:
- 5
- DOI:
- 10.3760/j.issn:1002-0098.2000.05.002
- Key Word:
- 牙周炎;中性白细胞;肿瘤坏死因子;白细胞介素1
Abstract： 目的 探讨快速进展性牙周炎(rapidly progressive periodontitis, RPP)患者局部牙周组织中多形核白细胞(polymorphonuclear neutrophil, PMN)过度浸润及激活与肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)和白细胞介素1β(interleukin-1β,IL-1β)的关系.方法 采用夹心ELISA法检测22例RPP患者共41个位点的龈沟液TNF-α水平,并采用酶促反应法检测同一份龈沟液中的PMN特异酶--弹性蛋白酶(elastase,EA)的活性,通过Spearman相关检验分析TNF-α水平与EA活性的相关性.另取10例RPP患者的牙龈组织,采用免疫组化法检测TNF-α和IL-1β蛋白的表达,通过HE染色观察PMN浸润.结果 龈沟液中的TNF-α水平[(3.23±1.81)ng/位点]与EA活性[(0.54±0.37)Abs/位点]呈显著正相关(r＝0.44,P＜0.05);牙龈组织中的TNF-α和IL-1β蛋白表达与PMN浸润密切相关;有大量PMN浸润的组织,炎症浸润广泛,袋上皮的增生与溃疡显著.结论 与TNF-α和IL-1β等细胞因子相关的PMN的过度浸润与激活促进了RPP的进展.
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