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Antitumor effect of radiation combined with tumor draining lymphocytes on human ACC-M cell in vitro
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNA OF STOMTAOLOGY
- Issue:
- 4
- DOI:
- 10.3760/j.issn:1002-0098.2000.04.001
- Key Word:
- 淋巴细胞;癌,腺样囊性
Abstract： 目的 探索放射联合肿瘤引流区淋巴结淋巴细胞(tumor draining lymph node lymphocyte, DNL)对肿瘤细胞的作用.方法 取首次治疗病理证实的口腔癌患者颈清扫淋巴结,经常规消化、分离,加白细胞介素2(interleukin-2, IL-2)培养,获取激活的淋巴细胞作为效应细胞,以效靶比(E∶T)25∶1进行集落形成试验,观察对人涎腺腺样囊性癌肺高转移细胞株(adenoid cystic carcinoma, ACC-M)的抗瘤效应,并分别以不同剂量60钴γ射线对ACC-M照射后,随即加入DNL细胞,观察放射结合DNL对ACC-M的抗瘤效应.结果 ACC-M为放射中度敏感细胞.根据线性--二次数学模型(LQ模型),其α、β值分别为0.342 0,0.078 3.DNL对ACC-M的抑瘤率为49.06% .放射联合DNL作用时,α值提高到0.768 8;Dq、S2分别从1.590 1、0.448 1降至0.599 5、0.113 5,这3个参数与放射组相比,差异均有显著性(P＜0.05).在高剂量区,与放射组比,放射联合淋巴细胞组的D0. 01和6 Gy、8 Gy剂量点的细胞存活率均明显下降,P＜0.01.结论 体外来源于不同病理类型口腔癌的IL-2激活的DNL对ACC-M有明显的抗瘤效应,无论在低剂量还是高剂量区与放射结合都有显著的协同抗瘤效应.
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