Abstract： Objective To investigate the association between tacrolimus binding protein 5 (FKBP5)gene polymorphisms and major depressive disorder.Methods A total of 254 major depressive disorder patients and 231 healthy controls were recruited.Two polymorphisms (rs3800373 and rs1360780) of FKBP5 gene were genotyped using the ligase detection reaction.Results The distribution frequencies of the SNP of rs3800373 showed no significant differences between patients ( GG 4.7％ ; GT 41.9％ ; TT 53.4％ ) and controls ( GG 6.6％ ; GT 38.2％ ; TT 55.3％ ) (x2 =1.252,P＞0.05).The distribution frequencies of the SNP of rs1360780 showed no significant differences between patients ( CC 55.4％ ; CT 39.9％ ;TT 4.7％ )and controls( CC 56.8％ ; CT 37.0％ ; TT 6.2％ ) (x2 =0.739,P＞0.05).No significant differences were found in allele frequencies of the SNPs of rs3800373 and rs1360780 between patients and controls (P＞0.05).No significant differences were found in rs3800373-rs1360780 haplotypes frequencies between patients and controls (P＞0.05).Following analyzing the clinical symptom phenotypes,no association was found between the rs3800373 and rs1360780 polymorphisms and the factors scores of Hamilton depression scale (HAMD),Hamilton anxiety scale (HAMA) and brief psychiatric rating scale (BPRS) ( P＞0.05).Conclusions The FKBP5 gene polymorphisms might not be associated with major depressive disorder.