Abstract： Objective To assess the clinical value of 99Tcm-tetrofosmin (TF) MPI for diagnosis of CHD.Methods A total of 73 patients (46 males,27 females; age:(61.16 ± 12.95) y) were included in this study.99Tcm-TF MPI and CAG were performed in all patients.Two-day protocol of MPI was used in this study,with rest MPI performed on the first day and stress (exercise stress or adenosine stress) imaging on the next day.CAG was performed within one week after MPI and served as the gold standard in diagnosis of CHD.Stenosis over 50％ of the lumen was considered positive.The diagnostic efficacy of 99Tcm-TF MPI was evaluated based on the results of CAG.Results Among the 73 patients,43 patients were CAG positive,27 cases with single vessel disease,10 patients with double vessel disease and 6 patients with triple vessel disease.There were 37 patients with abnormality in MPI and 36 patients with normal MPI.Out of 43patients with positive CAG,there were 32 cases diagnosed with myocardial ischemia and 11 with normal results in MPI.Among the 65 diseased vessels,there were 33 involved in LAD,16 LCX and 16 RCA.The sensitivity,specificity,positive predictive value,negative predictive value and accuracy of MPI for detection of CHD were 74.4％ (32/43),83.3％ (25/30),86.5％ (32/37),69.4％ (25/36) and 78.1％ (57/73),respectively.The sensitivities for detection of single vessel disease,double vessel disease and triple vessel disease were 66.7％ (18/27),80.0％ (8/10) and 5/6,respectively.The sensitivity and specificity of MPI for detection of coronary disease in all 219 vessels were 67.7％ (44/65) and 89.0％ (137/154),respectively.Conclusion 99Tcm-TF MPI,with convenient preparation and reliable properties of the tracer,may provide an alternative modality for accurate detection of CHD.