Abstract： Objective To investigate the effects of flight environmental factors,such as hypobaric hypoxia or +Gz exposure on the formation of kidney stone. Methods Forty-eight adult male Wistar rats were randomly and averagely assigned into control,hypobaric hypoxia, + Gz exposure and stone formation group.The rats of hypobaric hypoxia group were tested in 5000 m equivalent hypoxia environment for 1 h per 3 times a week for 4 weeks.+ Gz exposure group was exposed in +9 Gz for 25 s,5 times/d (with 5 min interval) by centrifuge.The frequency of +Gz exposure was 3 times a week for 4 weeks.The stone formation group was fed with water with 1％ ethylene glycol and poured with 2％ ammonium chloride by the dose of 2 ml/d for each.The serum blood urea nitrogen (BUN),creatinine (Scr),phosphorus(Pi),Ca2+,24 h urine volume,urinary excretion of Ca2+,oxalare (Ox),uric acid and urine pH value were detected.All rats' kidneys were removed and stained with hematoxylin-eosin (HE),and then examined by microscope to check the possible calculi crystals and tubular dilatation. Results ① The concentrations of BUN in + Gz exposure group and stone formation group were higher than that of control group (P＜0.05).Other data showed no difference among all groups. ② 24 h urine volume and Ca2+ of urine were no significant difference among groups but the uric acid (F=33.064,P＜0.01).Uric acid of urine of +Gz exposure group and stone formation group was significantly higher than that of control group (P＜0.01).Significant difference on oxalate of urine was observed among all groups (F=4.877,P＜0.01).Oxalate of urine of +Gz exposure group and stone formation group was higher than that of control group (P＜0.05).Urine pH value of hypobaric hypoxia group was lower than that of control group (t=3.099,P＜0.05).③ Renal tubules were normal and no calculi crystal was found in control group.Renal tubules were normal and only slight amount of calculi crystals were found in hypobaric hypoxia group.In +Gz exposure group,transparent scattered crystals could be seen and the tubules were slightly dilated.Big amount of transparent crystals could be seen in lumen of the evidently dilated tubules in stone formation group. Conclusions Acceleration would be one of the risk factors contributed to kidney stone formation.But the effect of hypobaric hypoxia on the formation of kidney stone is not obvious.