Monitoring and analysis of airborne microbial pollution in the simutated 480 m saturation diving chamber

( views:171, downloads:0 )
Author:
CHEN Shuang-hong(Department of Ship Sanitation, Naval Medical Research Institute, Shanghai 200433, China)
CHEN Rui-yong()
XIAO Cun-jie(Department of Ship Sanitation, Naval Medical Research Institute, Shanghai 200433, China)
TIAN Li()
XU Xiong-li(Department of Ship Sanitation, Naval Medical Research Institute, Shanghai 200433, China)
WU Wen-bin(Department of Ship Sanitation, Naval Medical Research Institute, Shanghai 200433, China)
BA Jian-bo(Department of Ship Sanitation, Naval Medical Research Institute, Shanghai 200433, China)
Journal Title:
Chinese Journal of Nautical Medicine and Hyperbaric Medicine
Issue:
Volume 19, Issue 02, 2012
DOI:
10.3760/cma.j.issn.1009-6906.2012.02.004
Key Word:
Saturation diving chamber;Airborne microbes;Monitoring;Evaluation

Abstract: Objective To monitor and evaluate the airborne microbial pollution in the confined environment of the 480 m saturation diving chamber and to provide evidences for a reliable evaluation of the health hazard caused by microbes in the chamber environment,as well as for the development of scientific intervention measures.Methods The level of microbes in the environment was measured with the natural sedimentation method,the growing features of indicator microbes were screened with selected medium,types of isolated strains were identified with the VITKE2 system,and airbome microbial contents in the chamber were evaluated with the General National Indoor Microbe Pollution Evaluation Standards.The effect of the environment on the related biological features of the virulence of Pseudomonas aeruginosa,the dominant pathogen in the chamber was monitored by means of the growing curve and motor ability.Results As measured in the experiment,the level of airborne microbes in the saturation diving chamber was 3.9 - 8.4 times higher than that of the national evaluation standards.Microbial types were dominantly human origin pathogens.The growth and motor ability of the prevailing Pseudomonas aeruginosa were enhanced,when compared to the control groups.Conclusions Microbial pollution in the 480 m saturation diving chamber was serious,heliox saturation could induce or enhance the virulence of Pseudomonas aeruginosa,and pathogenic microbes were a potential health hazard to divers in the hyperbaric environment.

  • [1]于玺华,车风翔.现代空气微生物学及采检鉴技术.北京:军事医学科学出版社,1998:175-260.
  • [2]国家质量监督检验检疫局,国家环保总局,国家卫生部.GB/T 188832.2002.室内空气质量标准.北京:中国标准出版社,2002.
  • [3]Murray PR,Barron EJ,Pfaller MA,等.临床微生物学手册.徐建新,梁国栋,刑来君,等译.北京:科学出版社,2005:737-748.
  • [4]Ahlen C. An in-field demonstration of the true relationship between skin infections and their sources in occupational diving systems in the North Sea.Ann Occup Hyg,2003,47(3):227-233.
  • [5]Goodman AL,Kulasekara B,Rietsch A,et al.A signaling network reciprocally regulates genes associated with acute infection and chronic persistence in Pseudomonas aeruginosa.Develop Cell,2004,7(5):745-751.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn