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Effect of HBO on ultrastructures of cerebrovascular anterior and posterior circulation system and ET-1 and eNOS in rabbits

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Author:
No author available
Journal Title:
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
Issue:
3
DOI:
10.3760/cma.j.issn.1009-6906.2011.03.010
Key Word:
高压氧;颈内动脉;基底动脉;内皮素-1;内皮型一氧化氮合酶;超微结构;Hyperbaric oxygen;Internal carotid artery;Basal artery;Endothelin-1;Nitric oxide synthase;Ultrastructure

Abstract: Objective To investigate changes in the ultrastructures and expression of endothelial nitric oxide synthase (eNOS) and endothelin-1 (ET-1) of internal carotid artery (ICA) and basal artery (BA) following hyperbaric oxygen (HBO).Methods Twenty rabbits were randomly divided into 2 groups: the experimental group and the control group. The rabbits in the experimental group were exposed to HBO at 2.2 ATA for 60 minutes for 3 days. The rabbits in the control group were normally fed without any treatment. The expressions of eNOS, ET-1 of ICA and BA in the 2 groups were compared by using immunohistochemical technique. Then, changes in the ICA and BA ultrastructures of rabbits in the two groups were examined under electron microscope.Results The expression of ET-1 in ICA was significantly higher than that in BA, while that of eNOS in BA was more intensive than that in ICA. When a comparison was made between the experimental group and the control group, the internal elastic membrane of ICA for the former was loosened, lipid vacuolization was present, and endothelial cells and organelles degenerated slightly. Nevertheless, no significant changes were noted in the ultrastructures of BA.Conclusions The expression of ET-1 in ICA increased and the expression of eNOS in BA intensified following HBO therapy. Changes in the ultrastructures might be correlated with vasoconstriction, reduction of blood flow and accumulation of lipid peroxides.

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