Abstract： Objective To develop an animal model of acute renal lesion induced by open abdominal injury coupled with seawater immersion, so as to provide a technical platform for further researches on injuries sustained during naval warfare. Methods Seventy-two male healthy Wister rots were randomly divided into 3 groups: Group A, the normal group; Group B, the pure abdominal open injury group; Group C, the abdominal open injury - seawater immersion group. Vital signs, body temperature, BUN (blood urea nitrogen), serum creatinine (Set), electrolytes (Na~+, K~+ , Cl~-) and CO_2CP were monitored to observe pathological changes in HE-pigmentation kidney. Results Acute renal lesion occurred to all the animals with abdominal open injury following a 3-h seawater immersion, when a eomparison was made with the normal group and the pure abdominal open injury group, displaying high Na~+ , K~+ , Cl~-, and metabolic acidosis. At the same time, Scr and BUN elevated significantly (P < 0. 05) and renal pathology showed that there were acute pathological changes. Conclusions Open abdominal injury coupled with a 3-h seawater immersion would induce acute renal lesion. This animal model had the features of combat injury complicated with seawater immersion, and at the same time it was economical and easy to reproduce. For this reason, it could offer a solid base for further research on acute renal lesion induced by seawater immersion following open abdominal injury.