Expression of TLR4 gene in mice exposed to 230 kPa hyperbaric oxygen during different time courses

( views:, downloads: )
MA Jun(Department of Diving Medicine, Faculty of Navy Medicine, Second Military Medical University, Shanghai 200433, China)
FANG Yi-qun()
MENG Miao()
YUAN Heng-rong()
WANG Yan()
Journal Title:
Volume 17, Issue 01, 2010
Key Word:
Pulmonary oxygen toxicity; Toll-like receptor 4; Acute lung injury

Abstract: Objective To investigate the expression of TLR4 gene in mice exposed to 230 kPa hyperbaric oxygen during different time courses, and the role of TLR4 in the development of pulmonary oxygen toxicity. Methods Forty C57BL/6 mice were randomly divided into 4 groups: the control group(UPTD, unit pulmonary toxicity dose =0), the 2-h exposure to 230 kPa oxygen group (UPTD =347), the 6-h group (UPTD = 1044), the 10-h group (UPTD = 1740), the norm oxygen group (hyperbaric and normal O_2 density group). Pathologic changes in the pulmonary tissue were observed and weight ratio of wet lung to dry lung and the expression of TLR4 were measured in vivo by using applied quantitative real time PCR. Results Pulmonary collapse hemorrhage, congestion and leucocyte aggregation in lung interstitium were observed in mice exposed to pure oxygen. Conclusions Lesion of the pulmonary tissue in mice deteriorated gradually with the extension of exposure. Highly negative correlation between TLR4 and UPTD indicated that TLR4 might be involved in the development of pulmonary oxygen toxicity, whose mechanism remained to be explored.

  • [1]龚锦涵.潜水医学.北京:人民军医出版社,1985:288-309.
  • [2]Armstrong L,Medford AR,Uppington KM,et al.Expression of functional toll-like receptoro2 and -4 on alveolar epithelial cells.Am J Respir Cell Mol Biol,2004,31(2):241-245.
  • [3]Hudak BB,Zhang LY,Kleeberger SR.Inter-strain variation in susceptibility to hyperoxic injury of murine airways.Pharmacogenetics,1993,85 (3):135-143.
  • [4]Cho HY,Jedlicka AE,Reddy SP,et al.Linkage analysis of susceptibility to hyperoxia:Nrf2 is a candidate gene.Am J Respir Cell Mol Biol,2002,26(1):42-51.
  • [5]马骏,孟淼,方以群.Bcl-2家族蛋白与肺型氧中毒.中华航海医学与高气压医学杂志,2008,15(5):316-318.
  • [6]Zhang X,Shan P,Qureshi S,et al.Cutting edge:TLR4 deficiency confers susceptibility to lethal oxidant lung injury.J Immunol,2005,175(8):4834-4838.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615