Changes in the level of PBEF in acute lung injury induced by seawater perfusion following open chest injury

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Author:
HU Xiao-hong(Department of Pediatric Medicine, Navy General Hospital,Beijing 100048, China)
DUAN Yun-you()
LI Yi()
XU Shi-xia(Department of Pediatric Medicine, Navy General Hospital,Beijing 100048, China)
HAN Zhi-hai()
XUE Zhi-qiang()
Journal Title:
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
Issue:
Volume 17, Issue 01, 2010
DOI:
10.3760/cma.j.issn.1009-6906.2010.01.003
Key Word:
Pre-B-cell colony-enhancing factor; Acute lung injury;Seawater perfusion; Open chest injury

Abstract: Objective To investigate changes in the level of pre-B-cell colony-enhancing factor (PBEF) and its possible effect on acute lung injury (ALI) induced by seawater perfusion following open chest injury in dogs. Methods Sixteen healthy adult dogs were randomly divided into the pure open chest injury group (the control group) and seawater perfusion after injury group (later simply referred to as the seawater group). Plasma, bronchoalveolar lavage fluid (BALF) and lung tissue samples were collected before seawater-perfusion (0 h) and 6 h after seawater perfusion. The PBEF protein levels in plasma, BALF and lung tissue were measured with enzyme-linked immunosorbent assay (ELISA), immunohistochemistry and Western blot. The expression of mRNA synthesis of coding PBEF in lung homogenates was evaluated with real-time RT-PCR. Results Compared with those of the control animals, PBEF levels significantly increased in the animals of the seawater group [plasma: (3014.16±883.47) ng/L versus (1060.94±251.08) ng/L (P < 0.05); lung tissue(Western blot) :(0.233)±0.037) versus (0.1541±0.013) ng/L,with statistical significance (P <0.05); PBEF mRNA :5.27±0.05 versus 0.65±0.12, with obvious statistical significance (P < 0.05)]. PBEF levels were positively correlated with plasma osmotic pressure, with a relative coefficient of 0.744 (P<0.05). Conclusions Seawater perfusion could elevate the level of PBEF in animals with ALI induced by seawater perfusion following open chest injury, indicating that it might be closely related with the high permeability of seawater. PBEF was clearly involved in the pathophysiological process of ALI, and might be a novel biomarker which could be used to identify ALI induced by seawater perfusion.

  • [1]李辉,鹿尔驯,虞积耀,等.胸部开放伤海水灌注致急性肺损伤的实验研究.中国危重病急救医学,2000,12(10):598-601.
  • [2]Samal B,Sun Y,Stearns G,et al.Cloning and characterization of the cDNA encoding,a novel human pre-B-cell colonyenhancing factor.Mol Cell Bio,1994,14 (2):1431-1437.
  • [3]Ye SQ,Simon BA,Maloney JP,et al.Pre-B-cell colonyenhancing factor as a potential novel biomarker in acute lung injury.Am J Respir Crit Care Med,2005,171 (4):361-370.
  • [4]孟继光,段蕴铀,薛志强,等.胸部开放伤后海水灌入胸腔致急性肺损伤犬模型的实验研究.中华航海医学与高气压医学杂志,2008,15(2):74-77.
  • [5]钱桂生.急性肺损伤和急性呼吸窘迫综合征研究现状与展望.解放军医学杂志,2003,28(2):97-101.
  • [6]Kitani T,Okuno S,Fujisawa H.Growth phase-dependent changes in the subcellular localization of pre-B-cell colonyenhancing factor.FEBS Lett,2003,544(12):74-78.
  • [7]Berndt J,Kloting N,Kralisch S,et al.Plasma visfatin concentrations and fat depot-specific mRNA expression in humans.Diabetes,2005,54(10):2911-2916.
  • [8]Ognjanovic S,Bao S,Yamamoto SY,et al.Genomic organization of the gene coding for human pre-B-cell colony enhancing factor and expression in human fetal membranes.J Mol Endocrinol,2001,26(2):107-117.
  • [9]McGlothlin JR,Gao L,Lavoie T,et al.Molecular cloning and characterization of canine pre-B-cell colony-enhancing factor.Biochem Genet,2005,43 (3-4):127-141.
  • [10]Ognjanovic S,Bryant-Greenwood GD.Pre-B-cell colonyenhancing factor,a novel cytokine of human fetal membranes.Am J Obstet Gynecol,2002,187(4):1051-1058.
  • [11]Jia SH,Li Y,Parodo J,et al.Pre-B-cell colony-enhancing factor inhibits neutrophil apoptesis in experimental inflammation and clinical sepsis.J Clin Invest,2004,113(9):1318-1327.
  • [12]Mukaicla N.Pathophysiological roles of interleukin-8/CXCL8 in pulmonary diseases.Am J Physiol Lung Cell Mol Physiol,2003,284(4):L566-L577.
  • [13]Gardai S,Whitlock BB,Helgason C,et al.Activatian of SHIP by NADPH oxidase-stimulated lyn leads to enhanced apoptosis in neutrophils.J Biol Chem,2002,277(7):5236-5246.
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