Changes of histopathology and blood viscosity in mini pigs with dysbaric osteonecrosis

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LI Ci(Naval Medical Research Institute, Shanghai 200433, China)
FANG Yi-qun(Naval Medical Research Institute, Shanghai 200433, China)
MENG Miao(Naval Medical Research Institute, Shanghai 200433, China)
FU Guo-ju(Naval Medical Research Institute, Shanghai 200433, China)
WANG Jie()
ZHU Xiang-qi(Naval Medical Research Institute, Shanghai 200433, China)
JIANG Gong-da()
Journal Title:
Volume 17, Issue 01, 2010
Key Word:
Mini pig; Dysbaric osteonecrosis; Platelet; Blood viscosity

Abstract: Objective To develop the dysharie osteonecrosis (DON) model in mini pigs induced by rapid decompression, following repeated hyperbaric exposures and observe changes in blood rheology in DON. Methods Twelve male mini pigs were exposed to compressed air of 0.45 MPa for 6 hours twice to three times a week and were then brought to the surface with linear decompression at a rate of 0.35 MPa/min. Blood viscosity was measured with cone-plate type viscosimeter, platelet count was made under the light microscope. Emission of computed tomography (ECT) and pathologic examination were performed for the evaluation of accumulated rate of 99Tc in the femur, following 3 months of repeated compression and decompression. Results ECT showed an increased accumulation of isotope in the femur, when a comparison was made between the experimental animals and the control. Osteopatholngy revealed changes of osteonecrosis in the femur. Platelet count decreased progressively with frequencies of pressure exposures, and meanwhile blood viscosity also increased progressively at a low shear rate. Conclusions Dysharie osteronecrosis was usually accompanied with bone ischemia. Platelet consumption and increased blood viscosity induced by decompression bubbles could be attributed to decreased blood flow in the bone, resulting in osteonecrosis.

  • [1]Davis TR,Holloway IT,Pooley J.Effect of exposure to compressed air and elevated oxygen levels on bone blood flow in the rabbit.Undersea Biomed Res,1990,17(3):201-211.
  • [2]田伍训,余绍宁,云昌全,等.同位素清除法研究不充分减压时小型猪股骨血流量的变化.生物医学工程学杂志,1997,14(I):26-29.
  • [3]朱祥祺,田伍训,姜德玺,等.反复高气压暴露引起动物减压性骨坏死的实验模型的建立.中国病理生理杂志,2000,16(6):574-576.
  • [4]Slichter SJ,Stegall P,Smith K,et al.Dysbaric osteonecrosis:a consequence of intravascular bubble formation,endothelial damage,and platelet thrombosis.I Lab Clin Med,1981,98 (4):568-590.
  • [5]Stegall PJ,Huang TW,Smith KH.The etiology of experimentally induced osteonecrosis.Undersea Biomed Res,1976,3:40-41.
  • [6]Pontier JM,Blatteau JE,Valiee N.Blood platelet count and severity of decompression sickness in rats after a provocative dive.Aviat Space Environ Med,2008,79(8):61-764.
  • [7]Pontier JM,Jimenez C,Blatteau JE.Blood platelet count and bubble formation after a dive to 30 msw for 30 min.Aviat Space Environ Med,2008,79(12):1096-1099.
  • [8]董晓雁,林桂珍,蒋雯,等.高血压患者微循环改变及血小板聚集性的关系.高血压杂志,1998,6(1):42-44.
  • [9]郭杰,孔祥永,郭新胜.脑梗死患者血小板聚集和血粘度相关性分析.新乡医学院学报,2000,17(5):393-394.
  • [10]于爱丽,侯爱萍.潜水致减压性骨坏死2例报告.工业卫生与职业病,2002,28(1):4.
  • [11]洪梅,姜树华,王海罡.潜水捕捞作业人员减压性骨坏死患病情况调查.中国公共卫生,2008,24(6):699.
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