Effects of early hyperbaric oxygen and hypobaric intervention on the levels of calcitonin gene related protein and endothelin in rats in hypobaric environment

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Author:
PAN Shu-yi(Navy Genenal Hospital, Beijing 100037, China)
PAN Xiao-wen(Navy Genenal Hospital, Beijing 100037, China)
YANG Chen(Navy Genenal Hospital, Beijing 100037, China)
LI Ming-xin(Navy Genenal Hospital, Beijing 100037, China)
ZHANG Yu(Navy Genenal Hospital, Beijing 100037, China)
MENG Xiang-en(Navy Genenal Hospital, Beijing 100037, China)
LIU Wei(Navy Genenal Hospital, Beijing 100037, China)
ZHONG Wen()
LI Bao-guo()
Journal Title:
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
Issue:
Volume 15, Issue 06, 2008
DOI:
Key Word:
Hyperbaric oxygen; Altitude disease; Hypobaria; Calcitonin gene related protein; Endothelin

Abstract: Objective To investigate effects of early hyperbaric oxygen and hypobaric intervention on the levels of calcitonin gene related protein (CGRP) and endothelin ( ET) in rats in hypobaric environment, and to compare the effects of two methods. Methods The experimental Wistar rats were randomly divided into six groups: (1) Control group, (2) 3800 m(2 h) group, (3) 3800 m (3 d) group, (4) 3800 m (7 d) group, (5) Hyperbaric oxygen intervention group, (6) Hypobaric environment intervention group. Firstly, the rats in the second, third and fourth groups were placed in a hypobaric oxygen chamber, in which the pressure was decreased at equal speed from 0. 1 MPa to 0. 062 MPa, which corresponded the pressure at the 3800 m altitude. After two hours and the 3rd, 7th days, the rats were decapitated to obtain blood for later examination. Secondly, the rats in the fifth group received hyperbaric oxygen treatment (0. 2 MPa, once per day, oxygen inhalation for 60 minutes, successively 5 days). On the sixth day, the rats were placed in a hypobaric oxygen chamber, in which the pressure was decreased at equal speed to 0. 062 MPa. After two hours, the rats were decapitated to obtain blood for later examination. Thirdly, the rats in the sixth group received hypobaric hypoxia treatment (0.062 MPa, once per day, 2 h once, for successively 5 days). On the sixth day, the rats were placed in a hypobarie oxygen chamber, in which the pressure was decreased from equal speed to 0. 062 MPa. After two hours, the rats were decapitated to obtain blood for later examination. The blood samples obtained from above different treatment methods were centrifuged to obtain the supernatant, and the levels of CGRP and ET were measured by radioimmunology method. Results Compared with control group, CGRP level decreased in tendency with time extension, but there was no significant difference between of them. The hyperbaric oxygen treatment had no effect on CGRP level, but hypobaric hypoxia pretreatment can decrease CGRP level more obviously, but there was no significant difference between of them either. ET level increased in tendency with time extension, but there was no significant difference between of them. However, EF level significantly increased (P<0.05) after hyperbaric oxygen[ (305. 26 ±39. 66) μg/L] and hypobaric hypoxia pretreatment [ (309. 18 ± 25. 07) μg/L]. Conclusions Early intervention of hyperbaric oxygen and hypobarie hypoxia mainly influences ET level in rats, and hyperbaric oxygen pretreatment might enhance body adaptive capacity for plateau.

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