Pilot research of the expression of MMP-2 gene and Survivin gene in primary esophageal cancer and subclinical microscopic tumor

( views:120, downloads:0 )
WU Feng-peng(Department of Radiotherapy,First Hospital,Beijing University)
GAO Xian-shu(Department of Radiotherapy,First Hospital,Beijing University)
WANG Ya-di(Department of Radiotherapy,Fourth Hospital,Hebei Medical University,Shijiazhuang 050011,China)
LU Fu-he(Department of Radiotherapy,Fourth Hospital,Hebei Medical University,Shijiazhuang 050011,China)
DONG Zhi-ming()
QIAO Xue-ying(Department of Radiotherapy,Fourth Hospital,Hebei Medical University,Shijiazhuang 050011,China)
Journal Title:
Volume 17, Issue 04, 2008
Key Word:
Esophageal neoplasms;Subclinical microscopic tumor;Cancerous peripheral tissue;Gene expression

Abstract: Objective To examine the expression of MMP-2 gene and Survivin gene in subclinical microscopic tumor and its peripheral normal esophageal tissues,and study the radiation target in molecular level. Methods Esophageal squamous cancer and its peripheral tissue samples of 34 patients were cut into sequential sections.The expression of MMP-2 gene and Survivin gene then examined.The length of the peripheral esophageal tissue,positively expressing the two genes,was measured,and the relation among the experimental date,tumor stage and vertical length of tumor were analyzed. Results For tumor tissue,subclinical microscopic tumor and the peripheral differentiated normal tissue,the positive expression rate of MMP-2 was 85%,83%and 79%,respectively.The positive expression rate of Survivin was 76%,85%and 85%,respectively.The positive expression level of both MMP-2 and Survivin genes in subclinical microscopic tumor was significantly higher than that in the peripheral differentiated normal tissue(χ2=6.46,P=0.028 and χ2=16.15,P=0.001).The length was 17.2-70.4 mm and 15.0-82.4 mm of cancerous peripheral tissue with positive expression of MMP-2 gene upside and downside of the tumor.The length was<70 mm in 97% of the samples.For Survivin gene.the length was 3.7-76.4 mm and 16.1-56.3 mm.and was<70 mm in 96%of the samples.The length of cancerous peripheral esophageal tissue expressing the two genes increased significantly along with tumor stage or tumor length,and there was statistical correlation between the length of tumor and the positive expression ranges of Survivin gene. Conclusions Both MMP-2 gene and Survivin gene are positively expressed in esophageal cancerous peripheral tissue.The range positively expressing the two genes is<70 mm in more than 96%of the samples,and the length is correlated with the tumor stage.More attention should be paid to the peripheral differentiated normal tissue with positive expression of MMP-2 gene and Survivin gene in esophageal squamous carcinoma.

  • [1]Gao XS,Qiao XY,Wu FP,et al.Pathological analysis of clinical target volume margin for radiotherapy in patients with esophageal and gastroesophageal junction carcinoma.Int J Radiat Oncol Biol Phys,2007,67:389-396.
  • [2]Miller C.Carcinoma of thoracic oesophagus and cardia.A review of 405 cases.Br J Surg.1962,49:507-522.
  • [3]Elkon D,Lee MS,Hendrickson FR.Carcinoma of the esophagus:sites of reculTence and palliative benefits after definitive radiotherapy.Int J Radiat Oncol Biol Phys,1978,4(7-8):615-620.
  • [4]Herskovic A,Mart Z,al-Sarraf M,et al.Combined chemotherapy and radiotherapy compared with radiotherapy alone in patients with cancer ofthe esophagus.N Engl J Med,1992,326:1593-1598.
  • [5]Anrbrosini G,Adida C,Ahieri DC.Anovelanti2 apoptosisgene,survivin,expressed in cancer and lymphoma:NatMed,1997,3:917-921.
  • [6]Cooper js,Guo MD,Herskovic A,et al.Chemoradiotherapy of locally advanced esophageal cancer:long-term follow-up of a prospective randomized trial(RTOG 85-01).Radiation Therapy oncology Group.JAMA,1999,281:1623-1627.
  • [7]Minsky BD,Pajak TF,Ginsberg RJ,et al.INT 0123(Radiation Therapy Oncology Group 94-05)phaseⅢtrial of combined-modality therapy for esophageal cancer:high-dose versus standarddose radiation therapy.J Clin Oncol,2002,20:1167-1174.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn