You Position: Home > Paper
Radiation enhancement on fibrosarcoma cells by arsenic trioxide
( views:204, downloads:16 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF RADIATION ONCOLOGY
- Issue:
- 6
- DOI:
- 10.3760/j.issn:1004-4221.2006.06.005
- Key Word:
- 三氧化二砷;细胞系,纤维肉瘤;放射增敏剂
Abstract： 目的 研究和探讨三氧化二砷(As2O3)是否对纤维肉瘤细胞有放射增敏作用.方法 以人纤维肉瘤细胞HT1080为实验对象,首先检测As2O3的单药毒性,确定IC10、IC50和IC90.放射增敏作用的实验分为空白对照组、单纯给药组、单纯照射组(包括1、2、4、6、8、10 Gy剂量)、照射前加药组(于照射前24h加入设定浓度的As2O3,药物作用24 h后进行照射)和照射后加药组(于照射后即刻加入设定浓度的As2O3,药物作用24 h).所有实验均重复3次.采用克隆形成分析法观察单纯照射和照射联合As2O3对细胞的杀伤作用.计算细胞的存活分数,用多靶单击模型进行拟合并做图.结果 HT1080细胞的IC10、IC50和IC90剂量分别为0.57、3.67和12.0 μmol/L.无毒剂量的As2O3照射前给药增敏比(SER)为0.86(Do值比)、0.98(SF2值比),照射后给药SER为0.99(Do值比)、1.09(SF2值比).IC50剂量的As2O3照射前给药SER为0.90(Do值比)、0.87(SF2值比),照射后给药SER为1.14(Do值比)、1.08(SF2值比).IC90剂量的As2O3照射前给药和照射后给药的SER均为1.14(Do值比)、3.20(SF2值比),As2O3对低剂量照射的放射增敏作用好于高剂量照射(SERsF2＞SERDo).结论 As2O3对HT1080纤维肉瘤细胞具有一定的放射增敏作用,为临床放疗和As2O3联合应用提供了实验依据.
- [1]聂林,张洹,李萍,等.三氧化二砷提高恶性淋巴瘤细胞放射敏感性的观察[J].2005,(1).
- [2]张大昕,王兵,胡雷光.三氧化二砷对卵巢癌细胞的放射增强作用[J].2003,(1).
- [3]刘连新,朱安龙,陈炜,等.三氧化二砷对原发性肝癌的作用及其机理研究[J].2005,(1).
- [4]SharonW.Weiss.软组织肿瘤[M].2002.
- [5]J G, Seol,W H, Park,E S, Kim,etc.Effect of arsenic trioxide on cell cycle arrest in head and neck cancer cell line PCI-1.[J].1999,265(2).
- [6]Wang, Cun-Yu,Mayo, Marty W..TNF- and cancer therapy-induced apoptosis: Potentiation by inhibition of NF-kappaB.[J].1996,274(5288).
- [7]Karstens JH,Raab R,Wittekind C,etc.Preoperative versus postoperative chemoradiotherapy for rectal cancer.[J].2004,351(17).
- [8]孙鸿德,马玲,胡晓晨,等.癌灵Ⅰ号结合中医辨证治疗急性早幼粒细胞白血病32例[J].1992,(3).
- [9]I.C. Gibbs,G. Harsh,L. Tupper,etc.Phase I Trial of Arsenic Trioxide and Fractionated Stereotactic Radiation for Recurrent Malignant Glioma[J].2005,63(1).
- [10]Tsien RY.,Natoli G.,Karin M.,etc.Inhibition of NF-kappa B activation by arsenite through reaction with a critical cysteine in the activation loop of I kappa B kinase[J].2000,275(46).
- [11]Lee KM,Fong KW,Tan EH,etc.Randomized Trial of Radiotherapy Versus Concurrent Chemoradiotherapy Followed by Adjuvant Chemotherapy in Patients With American Joint Committee on Cancer/International Union Against Cancer Stage III and IV Nasopharyngeal Cancer of the Endemic Variet[J].2005,23(27).
- [12]Gunderson LL,Smalley SR,Hundahl SA,etc.Chemoradiotherapy after surgery compared with surgery alone for adenocarcinoma of the stomach or gastroesophageal junction.[J].2001,345(10).