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In vivo MR imaging of nanometer magnetically labeled bone marrow stromal cells transplanted via portal vein in rat liver
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF RADIOLOGY
- Issue:
- 2
- DOI:
- 10.3760/j.issn:1005-1201.2006.02.005
- Key Word:
- 干细胞;磁共振成像;细胞移植;门静脉;动物,实验
Abstract： 目的观察1.5 T场强MRI联合动物专用线圈是否可以活体示踪经门静脉移植的纳米级超顺磁性氧化铁颗粒标记的骨髓基质细胞(bone marrow stromal cells BMSCs),为介入性门静脉骨髓基质细胞移植治疗终末期肝脏疾病的研究提供进一步的依据.方法供体大鼠5只,梯度密度离心分离BMSCs,纳米级超顺磁性氧化铁颗粒和脂质体转染BMSCs,体外经普鲁士蓝染色和HE染色确定细胞标记率.受体大鼠15只,分为5组,分别为对照组和纳米级超顺磁性氧化铁颗粒标记的骨髓基质细胞经门静脉移植入正常大鼠肝脏后2 h、3 d、7 d及2周组.1.5 T场强MRI联合动物专用线圈行T1W、T2W和T2*序列扫描,观察肝脏信号改变情况,与对照组比较,并且与组织切片对照.结果纳米级超顺磁性氧化铁颗粒和脂质体转染BMSCs,细胞标记率>95%.经门静脉移植入正常大鼠肝脏后,T2*序列扫描显示经标记的BMSCs在肝内显示弥漫性的结节性低信号影,移植后2 h到2周均可见到细胞在受体肝脏内存在,组织学切片显示信号缺失部位与铁颗粒标记细胞相一致.结论纳米级超顺磁性铁氧体颗粒标记的大鼠BMSCs经门静脉移植后可以通过1.5 T场强行MRI活体示踪,为临床干细胞移植的应用提供可行的示踪方法.
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