MR diffusion tensor imaging in the evaluation of neural progenitor cells transplantation to acute injured canine spinal cord
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF RADIOLOGY
- Issue:
- 1
- DOI:
- 10.3760/j.issn:1005-1201.2006.01.006
- Key Word:
- 脊髓损伤;神经前体细胞;移植;磁共振成像
Abstract： 目的用MR扩散张量成像(diffusion tensor imaging,DTI)观察神经前体细胞移植对犬急性脊髓损伤的影响.方法经端粒酶转染人胚胎脑室下区(subventricular zone, SVZ)细胞建立永生化的神经前体细胞系,并转基因表达绿色荧光蛋白(green fluorescent protein, GFP)用于标记和示踪.制作犬T13脊髓左半横断损伤模型.8只犬于损伤后1周行细胞移植,移植点取脊髓半切损伤头侧和尾侧邻近区域的灰白质交界处,不用免疫抑制剂.分别于损伤前、损伤后1周、移植后1周(即损伤后2周)、移植后4周用DTI测量损伤侧和未损伤侧的表观扩散系数(apparent diffusion coefficient, ADC)值及部分各向异性(fractional anisotropy, FA)值,并对结果进行统计学分析.结果损伤前、损伤后1周、移植后1周、移植后4周损伤侧的ADC值分别为(1.00±0.15)、 (1.65±0.45)、 (1.44±0.48)、(1.43±0.26) ×10-3mm2/s,不同时间的差异有统计学意义(F=6.038,P=0.005);损伤侧的FA值分别为0.59±0.11、 0.30±0.17、 0.36±0.25、0.34±0.11,不同时间的差异有统计学意义(F=5.221,P=0.009).未损伤侧的ADC值分别为(1.01±0.17)、(1.32±0.06)、(1.10±0.24)、(1.14±0.22) ×10-3mm2/s,不同时间的差异无统计学意义(F=1.303,P=0.306);未损伤侧的FA值分别为0.60±0.09、0.38±0.25、0.46±0.15、0.50±0.21,不同时间的差异无统计学意义(F=2.797,P=0.072).结论 DTI对实验性脊髓损伤后脊髓损伤和修复过程的观察能提供有价值的信息.
- [1]王霄英,谭可,倪石磊,等.MRI和DTI评价犬急性脊髓损伤后的形态及功能改变[J].2004,(12).
- [2]Qu Y,Chakrabortty S,McDonald JW,etc.Embryonic stem cells differentiate into oligodendrocytes and myelinate in culture and after spinal cord transplantation.[J].2000,97(11).
- [3]Hackney DB,Schwartz ED.Diffusion-weighted MRI and the evaluation of spinal cord axonal integrity following injury and treatment.[J].2003,184(2).
- [4]Barker GJ.Diffusion-weighted imaging of the spinal cord and optic nerve.[J].2001,186(Suppl.1).
- [5]D A, Fenyes,P A, Narayana.In vivo diffusion tensor imaging of rat spinal cord with echo planar imaging.[J].1999,42(2).
- [6]Peter Jezzard,Alan S. Barnett,Carlo Pierpaoli.Characterization of and correction for eddy current artifacts in echo planar diffusion imaging[J].1998,39(5).
- [7]A M, Ulu?,D F, Moore,A S, Bojko,etc.Clinical use of diffusion-tensor imaging for diseases causing neuronal and axonal damage.[J].1900,20.
- [8]Bastin ME.Correction of eddy current-induced artefacts in diffusion tensor imaging using iterative cross-correlation.[J].1999,17(7).
- [9]Beaulieu C.The basis of anisotropic water diffusion in the nervous system - a technical review.[J].2002,15(7).
- [10]Hammell KW.Exploring quality of life following high spinal cord injury: a review and critique.[J].2004,42(9).
- [11]Mickey SK,Qu Y,Choi DW,etc.Transplanted embryonic stem cells survive, differentiate and promote recovery in injured rat spinal cord.[J].1999,5(12).
- [12]Murray M,Tessler A,Hackney DB,etc.Ex vivo MR determined apparent diffusion coefficients correlate with motor recovery mediated by intraspinal transplants of fibroblasts genetically modified to express BDNF.[J].2003,182(1).
- [13]Snyder EY,Jones LL,Tuszynski MH,etc.Neural stem cells constitutively secrete neurotrophic factors and promote extensive host axonal growth after spinal cord injury.[J].2003,181(2).
- [14]Clark CA,Werring DJ.Diffusion tensor imaging in spinal cord: methods and applications - a review.[J].2002,15(7).
- [15]Parker GJ,Hickman SJ,Symms MR,etc.Investigating cervical spinal cord structure using axial diffusion tensor imaging.[J].2002,16(1).
- [16]Le-Bihan D,Mangin JF,Poupon C,etc.Diffusion tensor imaging: concepts and applications.[J].2001,13(4).
- [17]Cao QL,Howard RM,Zhang YP,etc.Pluripotent stem cells engrafted into the normal or lesioned adult rat spinal cord are restricted to a glial lineage.[J].2001,167(1).