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Isolation,culture and intraportal transplantation of rat marrow stromal cell
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF RADIOLOGY
- Issue:
- 2
- DOI:
- 10.3760/j.issn:1005-1201.2004.02.004
- Key Word:
- 干细胞;细胞移植;肝硬化;放射学,介入性
Abstract： 目的观察骨髓基质细胞( marrow stromal cell,MSC)经门静脉移植后在同种异体大鼠体内的转归,为MSC体内诱导转化及功能发挥的研究提供基础.方法采用连续传代培养的方法纯化MSC,DAPI标记后经门静脉移植入受体,移植细胞数量为105个/只,20只受体大鼠分为4组,各组分别于移植后2 h、1周、2周、3周、4周处死,观察受体肝脏及肺脏内DAPI标记的移植细胞的分布和存活情况.结果体外培养的MSC具有良好的增殖能力,20只受体大鼠4只死亡,其余均存活至处死前,MSC经门静脉植入异体大鼠后2 h、1周、2周、3周、4周受体肝脏内均可见DAPI标记的移植细胞存活,而各期受体肺内未见DAPI标记的移植细胞存活.结论经门静脉注入MSC后,细胞主要分布于受体肝脏,在肝脏内分布呈现由门静脉小分支逐渐向肝实质内移行的过程,并与肝细胞紧密结合呈索状排列.
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