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Comparison of the biological features between human fetal hepatocyte and immortalized L-02 hepatocyte in vitro

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Author:
No author available
Journal Title:
CHINESE JOURNAL OF RADIOLOGY
Issue:
2
DOI:
10.3760/j.issn:1005-1201.2004.02.002
Key Word:
细胞移植;胎肝细胞;细胞,培养的;干细胞

Abstract: 目的对比体外培养的人胎肝细胞与L-02肝细胞形态学及增殖分化特征的异同,初步评价其用于移植的可行性,探讨介入性肝细胞移植术的理想供体来源.方法分离培养14~24周的引产胎儿肝细胞,放射免疫法测定上清液中甲胎蛋白(AFP)与白蛋白(ALB)含量,免疫细胞化学法检测细胞角蛋白19(CK-19)的表达.同法检测传代培养L-02肝细胞的蛋白表达.结果人胎肝细胞分离活率达95%,在体外存活最长达3周,可同时检测到AFP、ALB及CK-19表达,其中ALB分泌峰值42.06 μg/ml;L-02肝细胞增殖迅速,AFP与CK-19表达阴性,ALB表达在10 μg/ml水平,部分细胞多次传代后发生形态变异,ALB表达缺失.结论人胎肝细胞具有潜在的双向分化能力,是肝细胞移植较为合适的供体来源; L-02肝细胞不适用于移植.

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