The experimental study of VEGF antisense oligodeoxynucleotides with lipiodol in arterial embolization of liver cancer in rats
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF RADIOLOGY
- Issue:
- 12
- DOI:
- 10.3760/j.issn:1005-1201.2003.12.002
- Key Word:
- 寡聚脱氧核苷酸,反义;内皮生长因子;碘油;栓塞,治疗性;瘤256,Walker
Abstract： 目的观察血管内皮生长因子(vascular endothelial growth factor, VEGF)反义寡聚脱氧核苷酸(antisense oligodeoxynucleotides,asODN)抑制体外培养的Walker-256瘤细胞VEGF表达的情况,评价其与碘油混合行肝动脉栓塞对大鼠肝癌的治疗效果.方法将反义和正义VEGF寡核苷酸加入无血清培养的Walker-256瘤细胞的培养液中,48 h后酶联免疫吸附试验(ELISA)法测定上清VEGF含量,并观察上清刺激血管内皮细胞(ECV-304)生长受抑情况.30只Walker-256移植性大鼠肝癌模型数字法随机分成3组,分别经肝动脉注入超液态碘油(UFLP)0.2 ml(UFLP组,n=10),UFLP 0.2 ml+VEGF asODN 3 OD混合物(UFLP+asODN组,n=10),生理盐水0.2 ml (对照组,n=10).于术前及术后第7天行MR检查观察肿瘤的体积,用逆转录聚合酶链反应(RT-PCR)方法检测瘤内及瘤周VEGF mRNA含量,免疫组织化学法检测肿瘤组织 VEGF的表达及微血管密度(microvessel density, MVD).结果 VEGF asODN可以明显抑制培养的Walker-256瘤细胞VEGF的表达.动脉栓塞实验中,UFLP+asODN组肿瘤生长率明显低于UFLP组和对照组[分别为(140.1±33.8)%、(177.9±64.9)%和(403.9±69.4)%;F=60.02,P<0.01].瘤内及瘤周VEGF mRNA含量UFLP+asODN组最低,对照组其次,UFLP组最高.UFLP组、对照组、UFLP+asODN组肿瘤组织VEGF表达阳性率差异无显著性意义(分别为90%、70%、50%,P＞0.05),UFLP+asODN组肿瘤组织MVD值为53.1±18.4,明显低于对照组(73.2±20.4)和UFLP组(80.3±18.5)(F=5.44,P<0.05).结论 VEGF asODN可以抑制Walker-256瘤细胞VEGF的表达.与单纯碘油栓塞相比,VEGF asODN与碘油混合肝动脉栓塞可以明显降低肿瘤的生长率,减少肿瘤组织VEGF的表达及微血管密度.
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