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An experimental animal model of chronic myocardial hibernation: comparative study of cine-MR, myocardial single photon emission computed tomography and pathology
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF RADIOLOGY
- Issue:
- 6
- DOI:
- 10.3760/j.issn:1005-1201.2003.06.006
- Key Word:
- 心肌顿抑;多巴酚丁胺;磁共振成像;放射性核素显像
Abstract： 目的制作冬眠心肌的实验性动物模型,用于评估各种影像学方法鉴别冬眠心肌的价值及其他相关临床研究.方法中华小型猪13只, 全麻下左侧开胸,于回旋支近心段放置可进行性缩小的环缩环,30～50 d后行MR电影检查,分别在静息及小剂量多巴酚丁胺(5 μg*kg-1*min-1)负荷实验下观察左室各节段运动变化并计算其运动积分;同时在静息和硝酸异山梨酯负荷下完成核素(99m锝-甲氧基异丁基异腈,99mTc-MIBI)心肌灌注显像.随后处死动物行电镜取材及氯化三苯基四氮唑(TTC)染色.结果实验动物中3只于术中、术后3 d 及2周死亡,其余10只30 d后仍存活.其中 7只动物在静息下MR电影示左室侧壁及后下壁运动丧失或减弱,但在小剂量多巴酚丁胺负荷实验时,相应的节段运动增强.6只动物在静息下99mTc-MIBI心肌断层显像显示相应的心室节段心肌灌注明显或严重降低, 应用硝酸异山梨酯介入后心肌显像均有明显改善甚至恢复正常.电镜检查则提示缺血区有不同程度的存活心肌.结论采用环缩环能够在动物模型中复制低血流灌注的冬眠心肌.
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