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Bile leakage in transjugular intrahepatic portosystemic shunt: effect on growth and function of endothelium
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF RADIOLOGY
- Issue:
- 8
- DOI:
- 10.3760/j.issn:1005-1201.2002.08.007
- Key Word:
- 胆汁;内皮;细胞,培养的;体外研究
Abstract： 目的探讨胆汁对离体培养的内皮细胞生长及功能的影响,进一步了解胆汁对支架内皮化的影响.方法取人脐静脉内皮细胞进行体外培养,分别加入5%、10%、15%、20%、25%胆汁干预,观察内皮细胞生长状况,收获的细胞测总蛋白,四唑盐(MTT)吸光度值,条件培养液测血管性假性血友病因子(von Willebrand factor,简称 vWF)行内皮细胞鉴定及功能测定.结果含5%、10%、15%胆汁的细胞生长状况与不含胆汁者相似,含20%、25%胆汁的细胞明显减少并显幼稚;含25%胆汁的细胞MTT吸光度值及总蛋白较无胆汁者降低,差异有非常显著性意义(Kruskal-Wallis秩和检验,χ2=29.913,P=0.000 9及χ2=18.857,P=0.002);含20%以上浓度胆汁的细胞条件培养液中vWF含量也明显降低,差异有非常显著性意义(Kruskal-Wallis秩和检验,χ2=27.213,P=0.000 1).所收获的细胞vWF测定均阳性.结论一定浓度的胆汁具有抑制内皮细胞的生长及分泌vWF的功能.所收获的细胞vWF测定均阳性.结论一定浓度的胆汁具有抑制内皮细胞的生长及分泌vWF的功能.
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