Angiogenesis related gene expression profiles of human endometrial endothelial cells isolated from tissue of endometrial cancer

( views:384, downloads:0 )
DU Xue-lian(Department of Gynecologic Oncology, Shandong Cancer Hospital, Jinan 250117, China)
SHENG Xiu-gui(Department of Gynecologic Oncology, Shandong Cancer Hospital, Jinan 250117, China)
YAN Chun-xiao(Department of Gynecologic Oncology, Shandong Cancer Hospital, Jinan 250117, China)
LIU Ting(Department of Gynecologic Oncology, Shandong Cancer Hospital, Jinan 250117, China)
WANG Cong(Department of Gynecologic Oncology, Shandong Cancer Hospital, Jinan 250117, China)
SONG Qu-qing(Department of Gynecologic Oncology, Shandong Cancer Hospital, Jinan 250117, China)
LI Qing-shui(Department of Gynecologic Oncology, Shandong Cancer Hospital, Jinan 250117, China)
Journal Title:
Chinese Journal of Obstetrics and Gynecology
Volume 47, Issue 10, 2012
Key Word:
Endometrial neoplasms ; Endometrium ; Endothelial cells ; Neovascularization,pathologic; Oligonucleotide array sequence analysis

Abstract: Objective To identify and verify the different genes expression pattern between human endometrial endothelial cells (HEEC) isolated from endometrial cancer and normal endometrium.Methods Endothelial cells were isolated from 5 patients with endometrial cancer (endometrial cancer group 1) and 5 patients with normal endometria tissue (control group 1) admitted from June to November 2007 in Shandong Cancer Hospital.Global expression patterns of endothelial cells were examined using oligonucleotide microarrays.Tissues from 36 patients with endometrial cancer(endometrial cancer group 2) and 10 normal endometrial tissues (control group 2) admitted from January 2007 to April 2008 were selected to verify the expression of different genes,in which up-regulated genes including ESM1,MMP-10,SPP1 and HMGB1 were tested by quantitative real-time PCR and immunohistochemistry.Results Microarray analyses revealed 317 genes that exhibited > 2-fold or < 0.5 differences were identified (including 191 genes up-regulated and 126 down-regulated).Pathway analysis showed that these genes involved cell cycle,cell adhesion molecules,and extracellular matrix-receptor interaction were obviously predominant.Of them,97 up-regulated genes and 44 down-regulated genes were related to angiogenesis.The mRNA expression of ESM1,MMP-10,SPP1 and HMGB1 in endometrial cancer group 2 were 0.898、3.890、1.433 and 1.881,respectively.Positive expression of SPP1,MMP-10,ESM1 and HMGB1 was observed in endometrial cancer group 2.However,the SPP1,ESM1 and HMGB1 was negative expressed in control group 2.Conclusion It shows that there are the different angiogenesis related genes between endometrial cancer and normal endothelium,which will provide insights into the anti-angiogenesis therapy for endometrial cancers.

  • [1]Ruoslahti E,Rajotte D.An address system in the vasculature of normal tissues and tumors.Annu Rev Immunol,2000,18:813-827.
  • [2]杜雪莲,盛修贵,颜春晓,等.人子宫内膜癌血管内皮细胞的分离鉴定及生物学特性检测.中华肿瘤杂志,2012,34:409-412.
  • [3]Weil MR,Macatee T,Garner HR.Toward a universal standard:comparing two methods for standardizing spotted microarray data.Biotechniques,2002,32:1310-1314.
  • [4]Bednár M.DNA microarray technology and application.Med Sci Monit,2000,6:796-800.
  • [5]Livak KJ,Schmittgen TD.Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C (T)) Method.Methods,2001,25:402-408.
  • [6]Wu X,Zeng H,Zhang X,et al.Phosphatase of regenerating liver-3 promotes motility and metastasis of mouse melanoma cells.Am J Pathol,2004,164:2039-2054.
  • [7]Shijubo N,Uede T,Kon S,et al.Vascular endothelial growth factor and osteopontin in tumor biology.Crit Rev Oncog,2000,11:135-146.
  • [8]Takano S,Tsuboi K,Tomono Y,et al.Tissue factor,osteopontin,alphavbeta3 integrin expression in microvasculature of gliomas associated with vascular endothelial growth factor expression.Br J Cancer,2000,82:1967-1973.
  • [9]Tiniakos DG,Yu H,Liapis H.Osteopontin expression in ovarian carcinomas and tumors of low malignant potential (LMP).Hum Pathol,1998,29:1250-1254.
  • [10]Mirza M,Shaughnessy E,Hurley JK,et al.Osteopontin-c is a selective marker of breast cancer.Int J Cancer,2008,122:889-897.
  • [11]Hu Z,Lin D,Yuan J,et al.Overexpression of osteopontin is associated with more aggressive phenotypes in human non-small cell lung cancer.Clin Cancer Res,2005,11:4646-4652.
  • [12]Zeng Q,Si X,Horstmann H,et al.Prenylation-dependent association of protein-tyrosine phosphatases PRL-1,-2,and-3 with the plasma membrane and the early endosome.J Biol Chem,2000,275:21444-21452.
  • [13]Guo K,Li J,Tang JP,et al.Catalytic domain of PRL-3 plays an essential role in tumor metastasis:formation of PRL-3 tumors inside the blood vessels.Cancer Biol Ther,2004,3:945-951.
  • [14]Parker BS,Argani P,Cook BP,et al.Alterations in vascular gene expression in invasive breast carcinoma.Cancer Res,2004,64:7857-7866.
  • [15]Lin TS,Chiou SH,Wang LS,et al.Expression spectra of matrix metalloproteinases in metastatic non-small cell lung cancer.Oncol Rep,2004,12:717-723.
  • [16]Lu C,Bonome T,Li Y,et al.Gene alterations identified by expression profiling in tumor-associated endothelial cells from invasive ovarian carcinoma.Cancer Res,2007,67:1757-1768.
  • [17]Ljubimova JY,Fujita M,Khazenzon NM,et al.Changes in laminin isoforms associated with brain tumor invasion and angiogenesis.Front Biosci,2006,11:81-88.
  • [18]Athanassiadou P,Athanassiades P,Grapsa D,et al.The prognostic value of PTEN,p53,and beta-catenin in endometrial carcinoma:a prospective immunocytochemical study.Int J Gynecol Cancer,2007,17:697-704.
  • [19]Scholten AN,Aliredjo R,Creutzberg CL,et al.Combined Ecadherin,alpha-catenin,and beta-catenin expression is a favorable prognostic factor in endometrial carcinoma.Int J Gynecol Cancer,2006,16:1379-1385.
  • [20]Kirschmann DA,Seftor EA,Fong SF,et al.A molecular role for lysyl oxidase in breast cancer invasion.Cancer Res,2002,62:4478-4483.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615