Angiogenesis related gene expression profiles of human endometrial endothelial cells isolated from tissue of endometrial cancer

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Author:
DU Xue-lian(Department of Gynecologic Oncology, Shandong Cancer Hospital, Jinan 250117, China)
SHENG Xiu-gui(Department of Gynecologic Oncology, Shandong Cancer Hospital, Jinan 250117, China)
YAN Chun-xiao(Department of Gynecologic Oncology, Shandong Cancer Hospital, Jinan 250117, China)
LIU Ting(Department of Gynecologic Oncology, Shandong Cancer Hospital, Jinan 250117, China)
WANG Cong(Department of Gynecologic Oncology, Shandong Cancer Hospital, Jinan 250117, China)
SONG Qu-qing(Department of Gynecologic Oncology, Shandong Cancer Hospital, Jinan 250117, China)
LI Qing-shui(Department of Gynecologic Oncology, Shandong Cancer Hospital, Jinan 250117, China)
Journal Title:
Chinese Journal of Obstetrics and Gynecology
Issue:
Volume 47, Issue 10, 2012
DOI:
10.3760/cma.j.issn.0529-567x.2012.10.009
Key Word:
Endometrial neoplasms ; Endometrium ; Endothelial cells ; Neovascularization,pathologic; Oligonucleotide array sequence analysis

Abstract: Objective To identify and verify the different genes expression pattern between human endometrial endothelial cells (HEEC) isolated from endometrial cancer and normal endometrium.Methods Endothelial cells were isolated from 5 patients with endometrial cancer (endometrial cancer group 1) and 5 patients with normal endometria tissue (control group 1) admitted from June to November 2007 in Shandong Cancer Hospital.Global expression patterns of endothelial cells were examined using oligonucleotide microarrays.Tissues from 36 patients with endometrial cancer(endometrial cancer group 2) and 10 normal endometrial tissues (control group 2) admitted from January 2007 to April 2008 were selected to verify the expression of different genes,in which up-regulated genes including ESM1,MMP-10,SPP1 and HMGB1 were tested by quantitative real-time PCR and immunohistochemistry.Results Microarray analyses revealed 317 genes that exhibited > 2-fold or < 0.5 differences were identified (including 191 genes up-regulated and 126 down-regulated).Pathway analysis showed that these genes involved cell cycle,cell adhesion molecules,and extracellular matrix-receptor interaction were obviously predominant.Of them,97 up-regulated genes and 44 down-regulated genes were related to angiogenesis.The mRNA expression of ESM1,MMP-10,SPP1 and HMGB1 in endometrial cancer group 2 were 0.898、3.890、1.433 and 1.881,respectively.Positive expression of SPP1,MMP-10,ESM1 and HMGB1 was observed in endometrial cancer group 2.However,the SPP1,ESM1 and HMGB1 was negative expressed in control group 2.Conclusion It shows that there are the different angiogenesis related genes between endometrial cancer and normal endothelium,which will provide insights into the anti-angiogenesis therapy for endometrial cancers.

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