Association of methionine synthase reductase gene polymorphism with unexplained recurrent spontaneous abortion
- Author:
- No author available
- Journal Title:
- Chinese Journal of Obstetrics and Gynecology
- Issue:
- 10
- DOI:
- 10.3760/cma.j.issn.0529-567x.2012.10.006
- Key Word:
- 铁氧化还原蛋白NADP还原酶;流产,习惯性;多态现象,遗传;聚合酶链反应;Ferredoxin-NADP reductase;Abortion,habitual;Polymorphism,genetic;Polymerase chain reaction
Abstract： Objective To explore the relationship between the polymorphism of methionine synthase reductase(MTRR) A66G and the susceptibility to unexplained repeated spontaneous abortion (URSA).Methods Total of 200 Henan Han couples with URSA (URSA group) and 76 Henan Han healthy couples without URSA (control group)were enrolled in this study.Their MTRR A66G genotypes were determined by PCR restriction fragment length polymorphism (PCR-RFLP).Results (1) The allele frequencies of MTRR A66G:the frequencies of allele A and allele G in URSA group were 76.5％ (153/200)in husband and 72.8％ (146/200) in wife,23.5％ (47/200) in husband and 27.2％ (54/200) in wife,respectively.The frequencies of allele A and allele G in control group were 78.9％ (60/76) in husband and 78.3％ (59/76) in wife,21.1％ (16/76) in husband and 21.7％ (16/76) in wife,respectively.The frequencies of allele A and allele G were not significantly different between female and male subjects within the same experimental group (P ＞ 0.05),and also there were not significantly different between the same gender subjects at URAS and control groups(P ＞ 0.05).(2) The genotype frequencies of MTRR A66G:the frequencies of genotype AA,AG and GG in URSA group were 57.0％ (114/200) in husband and 52.0％ (104/200) in wife,39.0％ (78/200) in husband and 41.5％ (83/200) in wife,4.0％ (8/200) in husband and 6.5％ (13/200) in wife,prepectively.The frequencies of genotype AA,AG and GG in control group were 59.2％ (45/76) in husband and 59.2％ (50/76) in wife,39.5％ (30/76) in husband and 38.2％ (29/76) in wife;1.3 ％ (1/76) in husband and 2.6％ (2/76) in wife,prepectively.The frequencies of genotype AA,AG and GG were not significantly different between female and male subjects within the same group (P ＞ 0.05),and also there were not significantly different between the same gender subjects at URSA and control groups (P ＞0.05).(3) Combined genotype of couples:the combined genotype frequencies of GG + GG,GG + AG,GG +AA,AG + AG,AG + AA and AA + AA in URSA group were 1.0％ (2/200),2.5％ (5/200),6.0％ (12/200),20.0％ (40/200),38.0％ (76/200),and 32.5 ％ (65/200),prepectively ; the combined genotype frequencies in control group were 0,1.3％ (1/76),2.6％ (2/76),17.1％ (13/76),42.1％ (32/76),36.8％ (28/76),prepectively.The combined genotype analysis between the two groups were also not significantly different (P ＞ 0.05).Conclusion The polymorphism of MTRR A66G gene was not associated with the susceptibility to URSA (P ＞ 0.05),and so it was not the inherited genetic risk factor of URSA.
- [1]林其德.原因不明复发性流产的基础与临床研究进展[J].2003,(8).
- [2]笪伟,刘和俊.MTRR基因A66G多态性及血浆同型半胱氨酸水平与心肌梗死的关系[J].2011,(5).
- [3]杜子明,黄爱玲,柳息洪.人蛋氨酸合成酶还原酶A66G基因突变频率的检测[J].2004,(2).
- [4]李晓梅,张友忠,许燕雪,等.MTHFR基因C677T位点多态性与不明原因重复性自然流产的相关性[J].2004,(1).
- [5]Rosenblatt D S,Cooper B A,Pottier A,etc.Altered vitamin B12 metabolism in fibroblasts from a patient with megaloblastic anemia and homocystinuria due to a new defect in methionine biosynthesis.[J].1984.
- [6]D J, Gaughan,L A, Kluijtmans,S, Barbaux,etc.The methionine synthase reductase (MTRR) A66G polymorphism is a novel genetic determinant of plasma homocysteine concentrations.[J].2001,157(2).
- [7]Padmalatha S, Rai,T S, Murali,T G, Vasudevan,etc.Genetic variation in genes involved in folate and drug metabolism in a south Indian population.[J].2011,17 Suppl 1.
- [8]Zeev Blumenfeld,Benjamin Brenner.Thrombophilia-associated pregnancy wastage[J].1999,72(5).
- [9]Desouza Cyrus,McNamara DennisB,Keebler Mary,etc.Drugs affecting homocysteine metabolism: impact on cardiovascular risk.[J].2002,62(4).
- [10]Afman LA,van-der-Linden IJ,Kluijtmans LA,etc.The methionine synthase reductase 66A>G polymorphism is a maternal risk factor for spina bifida.[J].2006,84(12).
- [11]C. Steen,D. S. Rosenblatt,H. Scheying,etc.Cobalamin E (cblE) disease: A severe neurological disorder with megaloblastic anaemia, homocystinuria and low serum methionine[J].1997,20(5).
- [12]Matthews RG,Elmore CL.Defects in homocysteine metabolism: diversity among hyperhomocyst(e)inemias.[J].2007,45(12).
- [13]Cai, D.,Liu, X.,Pan, C.,etc.Association of polymorphisms in folate metabolic genes and prostate cancer risk: A case-control study in a Chinese population[J].2010,89(2).
- [14]Elmore CL,Lawrance AK,Rozen R,etc.Methionine synthase reductase deficiency results in adverse reproductive outcomes and congenital heart defects in mice.[J].2008,94(3).
- [15]Krupenko NI,Rozen R,Matthews RG,etc.Metabolic derangement of methionine and folate metabolism in mice deficient in methionine synthase reductase.[J].2007,91(1).
- [16]Swanson DA,Liu ML,Baker PJ,etc.Targeted disruption of the methionine synthase gene in mice.[J].2001,21(4).
- [17]Ludwig ML,Guenther BD,Rozen R,etc.The structure and properties of methylenetetrahydrofolate reductase from Escherichia coli suggest how folate ameliorates human hyperhomocysteinemia[J].1999,6(4).
- [18]Colson N,Csurhes PA,Tajouri L,etc.No association between MTHFR A1298C and MTRR A66G polymorphisms, and MS in an Australian cohort.[J].2007,252(1).
- [19]Sunden SLF,UNIV ILLINOIS IMMUNOL RESOURCE CTR URBANA IL 61801.,Park EI,etc.Betaine-homocysteine methyltransferase expression in porcine and human tissues and chromosomal localization of the human gene[J].1997,345(1).
- [20]Yamada K,Rozen R,Matthews RG,etc.Effects of common polymorphisms on the properties of recombinant human methylenetetrahydrofolate reductase.[J].2001,98(26).