Expression and significance of adipocyte fatty acid-binding protein in placenta, serum and umbilical cord blood in preeclampsia

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YAN Jian-ying(Department of Obstetrics and Gynecology, Fujian Maternity and Children Health Hospital, Fuzhou 350001, China)
WANG Xiao-juan()
Journal Title:
Volume 45, Issue 12, 2010
Key Word:
Pre-eclampsia;Fatty acid-binding proteins;Fetal blood;Placenta

Abstract: Objective To investigate the change of adipocyte fatty acid-binding protein(FABP4) in maternal serum and umbilical cord blood and FABP4 mRNA placental expression in patients with preeclampsia(PE). Methods A total of 60 women with PE and 60 normal pregnant women as control participated in this study. All are admitted to Fujian Maternity and Children Health Hospital for delivery from December 2008 to October 2009. Patients with PE were divided into early-onset group (n = 30, presented at ≤34 weeks of gestation) and late-onset group(n = 30, presented at > 34 weeks of gestation), with 30 normal pregnant women as early control group(≤34 weeks of gestation) and 30 as late control group(>34 weeks of gestation). Enzyme-linked immunosorbent assay (ELISA) was used to detect FABP4,fasting serum glucose,fasting insulin(FINS) in maternal serum and FABP4 in umbilical cord blood. Real-time fluorescent quantitative revere transcription PCR was used to detect placental FABP4 mRNA expression. Furthermore,clinical and biochemical parameters were recorded, such as body mass index(BMI), systolic pressure(SP),diastolic pressure (DP), mean arterial pressure (MAP), total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), high density lipoprotein (HDL), creatinine (Cr), uric acid (UA) , glomerular filtration rate (GFR), 24 hours urine protein in pregnant women and neonatal weight. Results (1) Maternal serum FABP4 was (176 ± 9) ng/L in early-onset PE group and (170 ± 9) ng/L in late-onset PE group, significantly elevated as compared to (81 ± 13) ng/L in early control group and (94 ± 15) ng/L in late control group. (2) Mean maternal FINS, homeostasis model of assessment for insulin resistence index (HOMA-IR) were significantly elevated in the early-onset PE group and late-onset PE group as compared to control groups, respectively. (3) Mean placental FABP4 mRNA expression were significantly elevated in the early-onset PE group and late-onset PE group as compared to late control group. However, no significant difference was found in placental FABP4 mRNA expression between early-onset and late-onset PE groups.(4) Mean umbilical cord blood FABP4 concentrations were significantly decreased in the early-onset PE group and late-onset PE group as compared to late control group. Furthermore, umbilical cord blood FABP4 concentration correlated negatively with maternal serum FABP4 level and placental FABP4 mRNA expression, but positively with neonatal weight. (5) Mean maternal serum FABP4 concentrations correlated positively with placental FABP4 mRNA expression,TG, FINS, HOMA-IR, Cr, UA; and negatively with HDL, GFR. Conclusions Increased FABP4 expression in maternal serum and placenta may be involved in the pathogenesis of preeclampsia. Increased FABP4 mRNA expression in placenta may contribute to high serum FABP4 level in women with PE.

  • [1]Zavalza-Gomez AB,Anaya-Prado R,Rincon-Sanchez AR,et al.Adipokines and insulin resistance during pregnancy.Diabetes Res Clin Pract,2008,80:8-15.
  • [2]乐杰.妇产科学.7版.北京:人民卫生出版社,2009:92-99.
  • [3]Magnussen EB,Vatten IJ,Lund-Nilsen TI,et al.Prepregnancy cardiovascular risk factors as predictors of pre-eclampsia:population based cohort study.BMJ,2007,335:978.
  • [4]Dukic A,Zivancevic-Simonovic S,Varjacic M,et al.Hyperlipidemia and pregnancy.Med Pregl,2009,62:80-84.
  • [5]Bayhan G,Kocyigit Y,Atamer A,et al.Potential atherogenic roles of lipids,lipoprotein (a) and lipid peroxidation in preeclampsia.Gynecol Endocrinol,2005,21:1-6.
  • [6]Scioscia M,Gumaa K,Rademacher TW.The link between insulin resistance and preeclampsia:new perspectives.J Reprod Immunol,2009,82:100-105.
  • [7]Stefanovic M,Vukomanovic P,Milosavljevic M,et al.Insulin resistance and C-reactive protein in preeclampsia.Bosn J Basic Med Sci,2009,9:235-238.
  • [8]Xu A,Wang Y,Xu JY,et al.Adipocyte fatty acid-binding protein is a plasma biomarker closely associated with obesity and metabolic syndrome.Clin Chem,2006,52:405-413.
  • [9]Boord.JB,Maeda K,Makowski L,et al.Combined adipocytemacrophage fatty acid-binding protein deficiency improves metabolism,atherosclerosis,and survival in apolipoprotein Edeficient mice.Circulation,2004,110:1492-1498.
  • [10]Fasshauer M,Seeger J,Waldeyer T,et al.Serum levels of the adipokine adipocyte fatty acid-binding protein are increased in preeclampsia.Am J Hypertens,2008,21:582-586.
  • [11]Shangguan X,Liu F,Wang H,et al.Alterations in serum adipocyte fatty acid binding protein and retinol binding protein-4 in normal pregnancy and preeclampsia.Clin Chim Acta,2009,407:58-61.
  • [12]Baar RA,Dingfelder CS,Smith LA,et al.Investigation of in vivo fatty acid metabolism in AFABP/aP2(-/-) mice.Am J Physiol Endocrinol Metab,2005,288:187-193.
  • [13]Furuhashi M,Tuncman G,Gorgun CZ,et al.Treatment of diabetes and atherosclerosis by inhibiting fatty-acid-binding protein aP2.Nature,2007,447:959-965.
  • [14]Tso AW,Xu A,Sham PC,et al.Serum adipocyte fatty acid binding protein as a new biomarker predicting the development of type 2 diabetes:a 10-year prospective study in a Chinese cohort.Diabetes Care,2007,30:2667-2672.
  • [15]Xu A,Tso AW,Cheung BM,et al.Circulating adipocyte-fatty acid binding protein levels predict the development of the metabolic syndrome:a 5-year prospective study.Circulation,2007,115:1537-1543.
  • [16]Kralisch S,Stepan H,Kratzsch J,et al.Serum levels of adipocyte fatty acid binding protein are increased in gestational diabetes mellitus.Eur J Endocrinol,2009,160:33-38.
  • [17]Sommer G,Ziegelmeier M,Bachmann A,et al.Serum levels of adipocyte fatty acid-binding protein (AFABP) are increased in chronic haemodialysis (CD).Clin Endocrinol (Oxf),2008,69:901-905.
  • [18]Biron-Shental T,Schaiff WT,Ratajczak CK,et al.Hypoxia regulates the expression of fatty acid-binding proteins in primary term human trophoblasts.Am J Obstet Gynecol,2007,197:511-516.
  • [19]Marinoni E,Corona G,Ciardo F,et al.Changes in the interrelationship between leptin,resistin and adiponectin in early neonatal life.Front Biosci,2010,1:52-58.
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