Efficiency comparison between two preimplantation genetic diagnostic methods for chromosomal translocation carriers

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Author:
XU Yan-wen(Reproductive Medicine Center,First Affiliated Hospital of Sun Yat-sen University,Gnangzhou 510080,China)
REN Xiu-lian(Reproductive Medicine Center,First Affiliated Hospital of Sun Yat-sen University,Gnangzhou 510080,China)
LIU Ying(Reproductive Medicine Center,First Affiliated Hospital of Sun Yat-sen University,Gnangzhou 510080,China)
ZENG Yan-hong(Reproductive Medicine Center,First Affiliated Hospital of Sun Yat-sen University,Gnangzhou 510080,China)
FANG Cong(Reproductive Medicine Center,First Affiliated Hospital of Sun Yat-sen University,Gnangzhou 510080,China)
GAO Ling(Reproductive Medicine Center,First Affiliated Hospital of Sun Yat-sen University,Gnangzhou 510080,China)
ZHOU Can-quan(Reproductive Medicine Center,First Affiliated Hospital of Sun Yat-sen University,Gnangzhou 510080,China)
ZHUANG Guang-lun(Reproductive Medicine Center,First Affiliated Hospital of Sun Yat-sen University,Gnangzhou 510080,China)
Journal Title:
CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
Issue:
Volume 43, Issue 08, 2008
DOI:
Key Word:
Preimplantation diagnosis;Translocation, genetic;Heterozygote;In situ hybridization, fluorescence

Abstract: Objective To compare the diagnostic efficiency between blastomere preimplantation genetic diagnosis (PGD) and polar body PGD for chromosomal translocation carriers. Methods Group A had 8 cycles using whole painting probes for the first polar body diagnosis, while group B had 29 cycles using two subtelomeric probes and one centromeric probe for the blastomere diagnosis. Results The fertilization rate of group A was significantly lower than group B [66. 1% (72/109) vs 85.2% (304/357) , P < 0.05]. There was no significant difference in the successful biopsy rate between two groups. However, group A had a significantly higher loss rate during fixation and higher no signal rate after fluorescence in situ hybridization [ FISH, 9. 6% (12/104) vs 1.6% (4/252), 11.2% (10/89) vs 3.0% (7/233) ]. Totally, the diagnostic efficiency in group A (72. 5% ,79/109 ) was significantly lower than that in group B( 89. 8%, 230/256, P < 0. 05 ). Although both the clinical pregnancy rate( 3/7 ) and implantation rate( 22. 2% ,4/18 ) of group A were higher, the differences were not statistically significant ( P > 0.05 ). Conclusion Both methods can be used efficiently in the PGD for chromosomal translocation carriers. Blastomere PGD has a higher diagnostic rate.

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