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Role of urotensin Ⅱ gene in the genetic susceptibility to gestational diabetes mellitus in northern Chinese women
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 11
- DOI:
- 10.3760/j.issn:0529-567X.2006.11.004
- Key Word:
- 糖尿病,妊娠;疾病遗传易感性;尿紧张素类;多态性,单核苷酸
Abstract： 目的 探讨尾加压素Ⅱ基因rs228648 位点单核苷酸多态性与妊娠期糖尿病(GDM)发病的关系.方法 应用聚合酶链反应-限制性内切酶片段长度多态性(PCR-RFLP) 技术;采用病例-对照研究的方法,对中国北方地区无血缘关系的70例GDM孕妇(GDM组)和70例正常妊娠妇女(对照组)的尾加压素Ⅱ基因rs228648位点 (G-A) 进行单核苷酸多态性分析.结果 (1)两组孕妇尾加压素Ⅱ基因rs228648位点各基因型频率分布均符合Hardy-Weinberg 平衡检验,具有群体代表性.(2)GDM组孕妇尾加压素Ⅱ基因rs228648 位点G等位基因频率为70.7%,对照组为57.9%,两组比较,差异有统计学意义(P＜0.05);GDM组孕妇尾加压素Ⅱ基因rs228648 位点A等位基因频率为29.3%,对照组为42.1%,两组比较,差异也有统计学意义(P＜0.05).而两组孕妇的G/G基因型频率比较(分别为52.9% 和 41.4%),差异无统计学意义(P》0.05).(3) 尾加压素Ⅱ基因rs228648位点的A/A基因型频率与GDM组呈负相关关系,经多因素logistic 回归分析显示,其OR 值为0.312,OR 值的95 %可信区间为0.108～0.900(P=0.031).结论 尾加压素Ⅱ基因rs228648 位点单核苷酸多态性可能在GDM遗传易感性中起重要作用.G 等位基因可能与GDM发生有关,而尾加压素Ⅱ基因rs228648位点的A型纯合子可能是GDM的重要保护因素.
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