Effects of estrogen receptor β on proliferation of ovarian clear cell adenocarcinoma ES-2 in vitro and in vivo
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 9
- DOI:
- 10.3760/j.issn:0529-567X.2006.09.004
- Key Word:
- 受体,雌激素;卵巢肿瘤;腺癌,透明细胞;细胞分裂;转染
Abstract： 目的 了解导入外源性人类全长雌激素受体(ER)β cDNA后,卵巢透明细胞癌细胞系ES-2生长情况和细胞周期的变化.方法 将带有Erβ cDNA的质粒pRSV-Erβ和对照空质粒pRSV分别转染ES-2细胞(分别命名为ES-pRSV-Erβ、ES-pRSV细胞),RT-PCR和蛋白印迹法检测ERβmRNA和蛋白的表达情况,四甲基偶氮唑蓝法、流式细胞仪监测ES-2、ES-pRSV、ES-pRSV-Erβ细胞在雌激素作用下的体外增殖能力、细胞周期的变化,并观察ES-2、ES-pRSV、ES-pRSV-Erβ细胞接种于裸鼠皮下的成瘤和种植瘤的生长情况,分别将裸鼠命名为ES-2、ES-pRSV和ES-pRSV-Erβ组.结果 ES-pRSV-Erβ细胞稳定表达Erβ mRNA和蛋白,而ES-pRSV和未转染的ES-2细胞均未检测出ERβmRNA和蛋白的表达条带.ES-pRSV-Erβ细胞在体外的增殖速度明显落后于未转染的ES-2和ES-pRSV细胞,雌激素作用7 d后,未转染的ES-2细胞的吸光度值为0.78±0.05,ES-pRSV细胞为0.81±0.06,两者比较,差异无统计学意义(P＞0.05);而ES-pRSV-Erβ细胞的吸光度值为0.53±0.07,明显低于ES-pRSV和未转染的ES-2细胞,差异有统计学意义(P＜0.01).裸鼠接种第24天,ES-pRSV-Erβ组的种植瘤平均体积为(2868±879)mm3,低于ES-2组的(3603±724)mm3和ES-pRSV组的(3913±624)mm3(P＜0.05).未转染的ES-2、ES-pRSV、ES-pRSV-Erβ细胞S期细胞所占比例分别为(37±9)%、(39±10)%、(20±5)%,ES-pRSV-Erβ细胞分别与未转染的ES-2、ES-pRSV细胞比较,差异有统计学意义(P＜0.05).结论 稳定表达Erβ的ES-2细胞体内、体外增殖能力明显减弱,Erβ以雌激素依赖方式使ES-2细胞周期中S期细胞比例减少,提示Erβ可能有肿瘤抑制作用.
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