Study on the relation between concentration of circulating non-host fetal DNA in pregnant women and pre-eclampsia
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 12
- DOI:
- 10.3760/j.issn:0529-567X.2005.12.005
- Key Word:
- DNA;先兆子痫;聚合酶链反应;内皮缩血管肽类
Abstract： 目的探讨孕妇外周血中胎儿DNA水平检测在子痫前期诊断中的应用价值.方法选择30例子痫前期孕妇为子痫前期组(其中轻度18例,重度12例),另选择30例正常孕妇作为对照组,两组孕妇分别于孕20周、孕晚期(子痫前期组孕33周+3、对照组孕34周+3)、分娩后1 、3 、 6 h取外周血,采用荧光定量PCR检测外周血中Y染色体上的性别决定基因(SRY基因)胎儿DNA水平(B超确定两组孕妇所妊娠的胎儿均为男性);放射免疫法检测两组孕妇孕晚期内皮素水平.结果(1)子痫前期组孕20周时的胎儿DNA水平为(316±61) copy/ml,其中轻度、重度患者分别为(266±79)、(396±91)copy/ml;对照组孕妇为(165±43)copy/ml,子痫前期组及轻度、重度患者明显高于对照组,两组比较,差异有统计学意义(P<0.01).(2)子痫前期组孕晚期胎儿DNA水平为(970±413) copy/ml, 其中轻度、重度患者分别为(758±357)、(1285±573)copy/ml,对照组孕妇为(319±99)copy/ml,子痫前期组及轻度、重度患者明显高于对照组,两组比较,差异有统计学意义(P<0.01).(3)子痫前期组产后1、3、6 h胎儿DNA水平分别为(139±45)、(76±31)、(44±13) copy/ml,其中轻度患者分别为(102±42)、(57±25)、(36±12 )copy/ml,重度患者分别为(209±51)、(97±40)、(52±17 )copy/ml;对照组分别为(33±13)、(9±5)、0 copy/ml.子痫前期组及轻度、重度患者明显高于对照组,两组比较,差异有统计学意义(P<0.01).(4)子痫前期组内皮素水平为(80±18)ng/L,其中轻度患者为(74±14)ng/L,重度患者为(89±32)ng/L;对照组为(50±11)ng/L,子痫前期组及轻度、重度患者明显高于对照组,两组比较,差异有统计学意义(P<0.01).(5)子痫前期组胎儿DNA水平与内皮素水平呈正相关关系( r=0.748,P<0.01 ).结论孕妇外周血胎儿DNA水平变化可以作为预测和诊断子痫前期发病与疾病程度的一个指标.
- [1]S C, Smith,P N, Baker,E M, Symonds.Placental apoptosis in normal human pregnancy.[J].1997,177(1).
- [2]乐杰.妇产科学[M].2004.
- [3]S, Chua,T, Wilkins,I, Sargent,etc.Trophoblast deportation in pre-eclamptic pregnancy.[J].1991,98.
- [4]Yoko, Ohashi,Norio, Miharu,Hiroshi, Honda,etc.Correlation of fetal DNA and human chorionic gonadotropin concentrations in second-trimester maternal serum.[J].2002,48(2).
- [5]Diana W. Bianchi.Fetal DNA in Maternal Plasma: The Plot Thickens and the Placental Barrier Thins[J].1998,62(4).
- [6]Farina A,Saito H,Sekizawa A,etc.Fetal DNA in maternal plasma as a screening variable for preeclampsia. A preliminary nonparametric analysis of detection rate in low-risk nonsymptomatic patients.[J].2004,24(2).
- [7]Lo YM,Corbetta N,Sargent IL,etc.Presence of fetal DNA in maternal plasma and serum.[J].1997,350(9076).
- [8]Sekizawa A,Samura O,Zhen DK,etc.Apoptosis in fetal nucleated erythrocytes circulating in maternal blood.[J].2000,20(11).
- [9]Ganshirt D,Holzgreve W,Maymon E,etc.Disturbed feto-maternal cell traffic in preeclampsia.[J].1998,91(5 Pt.1).
- [10]M, Smid,A, Vassallo,F, Lagona,etc.Quantitative analysis of fetal DNA in maternal plasma in pathological conditions associated with placental abnormalities.[J].2001,945.