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Primary study of vasculogenic mimicry induced by hypoxia in epithelial ovarian carcinoma
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 10
- DOI:
- 10.3760/j.issn:0529-567X.2005.10.005
- Key Word:
- 卵巢肿瘤;新生血管化,病理性;细胞缺氧;西罗莫司
Abstract： 目的探讨缺氧在卵巢上皮性癌细胞系SKOV3和ES2细胞拟态血管形成过程中的作用及西罗莫司对其的抑制效应.方法建立人工基底膜基质凝胶Matrigel三维培养系统,分别在常氧(常氧组)、缺氧(缺氧组)、缺氧时加入西罗莫司(缺氧+西罗莫司组)等条件下诱导,观察SKOV3和ES2细胞形成拟态血管的能力;应用相差显微镜和扫描电镜观察拟态血管的形态及其立体结构的特点;用RT-PCR技术检测不同条件下缺氧诱导因子(HIF)1α mRNA的相对表达量.结果培养7 d后,缺氧组SKOV3和ES2细胞出现变形、伸展,形成腔样、管状、分支和网络状等管道结构;而常氧组及缺氧+西罗莫司组,部分细胞出现伸展但不形成管道结构.缺氧组SKOV3和ES2细胞的HIF-1α mRNA表达量分别为0.801±0.034和0.736±0.059,显著高于缺氧+西罗莫司组(分别为0.025±0.007和0.231±0.035;P<0.01,P<0.05)和常氧组(分别为0.010±0.004和0.011±0.002; P均<0.01).结论缺氧是卵巢上皮性癌细胞形成拟态血管的重要诱导因素,西罗莫司通过抑制HIF-1αmRNA的表达阻断拟态血管的形成.
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