Clinical significance of KiSS-1 and matrix metalloproteinase-9 expression in trophoblasts of women with preeclampsia and their relation to perinatal outcome of neonates
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 9
- DOI:
- 10.3760/j.issn:0529-567X.2005.09.003
- Key Word:
- 先兆子痫;滋养层;蛋白质类;明胶酶B;妊娠结局
Abstract： 目的探讨胎盘滋养细胞中KiSS-1基因及基质金属蛋白酶(MMP)9在子痫前期发病中的作用及其与新生儿预后的相关性.方法采用RT- PCR和western blot(蛋白印迹法)检测40例子痫前期患者(其中轻度15例、重度25例,子痫前期组)和20例正常足月正常妊娠妇女(正常妊娠组)胎盘滋养细胞中KiSS-1 mRNA和MMP-9 mRNA 及其蛋白的表达,并与其临床症状及其新生儿围产结局进行相关性分析.结果 (1)子痫前期组滋养细胞中KiSS-1mRNA及其蛋白表达水平分别为1.73±0.24和(78.4±8.0) μg /100 μg总蛋白,其中轻度患者为1.50±0.15和(72.4±6.9) μg/100 μg总蛋白, 重度患者为1.87±0.20和(83.5±3.6) μg /100 μg总蛋白;均显著高于正常妊娠组的1.24±0.25和(63.4±2.7) μg /100 μg总蛋白(P<0.01).(2)子痫前期组MMP-9 mRNA及其蛋白表达水平分别为0.09±0.06和(9.6±4.3) μg /100 μg总蛋白,其中轻度患者为0.11±0.08和(10.0±3.2) μg /100 μg总蛋白, 重度患者为0.07±0.05和(7.8±2.0) μg /100 μg总蛋白;均显著低于正常妊娠组的0.17±0.10和(17.9±7.3) μg /100 μg总蛋白(P<0.01).(3)子痫前期组KiSS-1 mRNA及其蛋白表达水平与中心动脉压(MAP)和24 h尿蛋白定量呈正相关,r分别为0.610(P=0.023)、 0.713(P=0.011)和0.397( P=0.003)、0.638(P=0.002);与有、无眼底动脉痉挛呈显著正相关,r分别为0.499( P=0.000)和0.511(P=0.000).子痫前期组MMP-9 mRNA及其蛋白表达水平则与MAP、24 h尿蛋白定量及有、无眼底动脉痉挛呈显著负相关,r分别为0.561(P=0.042)、 0.571(P=0.022)、0.275(P=0.039)、 0.375 (P=0.048)、0.346(P=0.001)、 0.543(P=0.000).(4)子痫前期组MMP-9 mRNA及其蛋白表达水平与新生儿体重呈显著正相关,r分别为 0.651( P=0.000)和0.544(P=0.004);KiSS-1mRNA及其蛋白表达水平与其呈显著负相关,r分别为0.759( P=0.000)和0.865(P=0.000).(5)随着新生儿窒息程度加重,MMP-9 mRNA及其蛋白表达水平呈降低趋势,KiSS-1mRNA及其蛋白表达水平呈升高趋势(P均<0.05).结论 MMP-9和 KiSS-1表达失衡在子痫前期发病中起重要作用,且与新生儿预后密切相关.
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