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Study on the correlation of transforming growth factor β1 and its receptors with spontaneous abortion after in vitro fertilization and embryo transfer
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 5
- DOI:
- 10.3760/j.issn:0529-567X.2005.05.004
- Key Word:
- 转化生长因子β;受体;自然流产
Abstract： 目的了解转化生长因子β1(TGF-β1)及其受体(TβR)在绒毛及蜕膜组织中的表达,初步探讨TGF-β1与体外受精-胚胎移植(IVF-ET)后流产及自然流产的关系.方法应用免疫组化链霉菌抗生物素蛋白-过氧化物酶(SP)连接法,检测10例IVF-ET后流产患者(IVF-ET组)和20例早期自然流产患者(自然流产组)的绒毛及蜕膜组织中的TGF-β1及TβR(Ⅰ、Ⅱ型)表达情况,其表达水平以阳性染色部位的吸光度(A)值表示,并与20例人工流产者(对照组)进行比较.结果 TGF-β1及TβR在早孕绒毛滋养细胞、蜕膜组织腺体及间质细胞中均有阳性染色,3组表达部位相同.TGF-β1在IVF-ET组蜕膜及绒毛组织中表达水平(A值,中位数,下同)分别为0.167、0.199,自然流产组为0.198、0.201,对照组为0.277、0.274;TβR-Ⅰ在IVF-ET组蜕膜及绒毛组织中表达水平为0.144、0.150,自然流产组为0.202、0.201,对照组为0.238、0.281;TβR-Ⅱ在IVF-ET组蜕膜及绒毛组织中的表达水平为0.199、0.145,自然流产组为0.153、0.156,对照组为0.300、0.301.上述指标,IVF-ET、自然流产组分别与对照组比较,差异均有统计学意义(P<0.01).结论 TGF-β1及TβR在IVF-ET后流产及自然流产患者绒毛及蜕膜组织中的表达下降,在IVF-ET后流产发病中起重要作用,是导致自然流产的原因之一.
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