You Position: Home > Paper
Ovarian insulin resistance and insulin sensitizer effect on polycystic ovary syndrome
( views:519, downloads:118 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 12
- DOI:
- 10.3760/j.issn:0529-567X.2004.12.004
- Key Word:
- 多囊卵巢综合征;噻唑类;胰岛素抗药性
Abstract： 目的探讨多囊卵巢综合征(PCOS)患者卵巢局部胰岛素抵抗状态及胰岛素增敏剂--曲格列酮(troglitazone)对改善卵巢局部胰岛素抵抗的作用.方法收集11例PCOS患者(PCOS组)和排卵功能正常的33例输卵管性不孕患者(对照组)促排卵后卵巢黄素化颗粒细胞(卵巢细胞),进行体外培养,并经不同浓度的胰岛素及胰岛素样生长因子1(IGF-1)作用后,观察卵巢细胞糖原和DNA合成的变化,同时观察卵巢细胞胰岛素受体(IR)、IGF-1受体(IGF-1R)、胰岛素受体底物(IRS)的表达.结果 (1)不同浓度IGF-1作用后,PCOS组卵巢细胞的DNA合成增加约为对照组的2倍,曲格列酮可抑制IGF-1的这一作用.(2)胰岛素作用后,PCOS组卵巢细胞的糖原合成量与对照组比较,明显降低;而曲格列酮作用后,两组卵巢细胞中胰岛素促糖原合成作用均增加.(3)与对照组比较,PCOS组卵巢细胞IRS-1的表达(相对灰度,下同)升高,IRS-2表达降低.曲格列酮可降低IRS-1的表达水平,升高IRS-2的表达水平.结论 (1)PCOS患者存在卵巢局部的胰岛素抵抗现象.(2)卵巢局部胰岛素抵抗与PCOS患者卵巢高反应状态相关.(3)曲格列酮可增加卵巢细胞对胰岛素的敏感性.
- [1]郑志群,李美芝,林益川,等.罗格列酮对多囊卵巢综合征胰岛素抵抗及高雄激素血症的治疗研究[J].2002,(5).
- [2]Y, Lin,M, Fridstr?m,T, Hillensj?.Insulin stimulation of lactate accumulation in isolated human granulosa-luteal cells: a comparison between normal and polycystic ovaries.[J].1997,12.
- [3]Dunaif A,Book CB.Selective insulin resistance in the polycystic ovary syndrome.[J].1999,84(9).
- [4]Auchus RJ,Miller WL,Arlt W.Thiazolidinediones but not metformin directly inhibit the steroidogenic enzymes P450c17 and 3beta -hydroxysteroid dehydrogenase.[J].2001,276(20).
- [5]A, Dunaif.Insulin resistance and the polycystic ovary syndrome: mechanism and implications for pathogenesis.[J].1997,18.
- [6]S. Inoue,K. Fukuda,T. Kudara,etc.Augmented growth response to IGF-1 via increased IRS-1 in Chinese hamster ovary cells expressing kinase-negative insulin receptors[J].1999,42(6).