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Expression of hypoxia inducible factor-1α in placenta of pregnancy induced hypertension
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 7
- DOI:
- 10.3760/j.issn:0529-567X.2004.07.004
- Key Word:
- 妊娠并发症,心血管;高血压;转录因子
Abstract： 目的研究缺氧诱导因子1α在妊娠高血压综合征(妊高征)患者胎盘组织中的表达,及其在妊高征发病机制中的作用.方法采用组织芯片技术和免疫组化方法检测49例妊高征患者(妊高征1组)及26例正常孕妇(对照1组)的胎盘组织中缺氧诱导因子1α蛋白的表达.采用RT-PCR技术检测12例(从妊高征1组中选出)妊高征患者(妊高征2组)及12例(从对照1组中选出)正常孕妇(对照2组)的胎盘组织中缺氧诱导因子1α mRNA的表达.结果 (1)妊高征1组缺氧诱导因子1α蛋白的阳性表达率为63.1%、中度阳性表达率为23.3%,显著高于对照1组缺氧诱导因子1α阳性表达率的20.9%、中度阳性表达率的7.0%(P<0.01).(2)妊高征2组缺氧诱导因子1α mRNA表达水平为0.96±0.37,对照2组为0.95±0.20,两组比较,差异无显著性 (P>0.05).结论(1)妊高征患者胎盘组织中缺氧诱导因子1α mRNA的表达水平与正常胎盘组织相似,但缺氧诱导因子1α蛋白水平明显高于正常胎盘组织.(2)缺氧诱导因子1α通过蛋白水平上的调节参与妊高征病理生理过程,而不是在转录水平.
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