You Position: Home > Paper
Study on the mechanism of intrauterine infection of hepatitis B virus
( views:295, downloads:280 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 4
- DOI:
- 10.3760/j.issn:0529-567X.2004.04.003
- Key Word:
- 肝炎,乙型;疾病传播,垂直
Abstract： 目的探讨乙型肝炎病毒(HBV)宫内感染的可能机理.方法应用PCR技术检测59例乙型肝炎病毒表面抗原(HBsAg)阳性孕妇的羊水、阴道分泌物及其新生儿脐血清中HBV DNA(研究组),10例乙型肝炎病毒标志物(HBVM)阴性的正常孕妇及其新生儿为对照组.采用免疫组化ABC染色法检测两组孕妇胎盘组织中的HBsAg及乙型肝炎核心抗原(HBcAg)的阳性率.结果 (1)研究组孕妇的羊水、阴道分泌物、新生儿脐血清中HBV DNA阳性率分别为 47.5%(28/59)、52.5%(31/59)、45.8%(27/59);对照组孕妇的羊水、阴道分泌物、新生儿脐血清中均未检出HBV DNA.(2)研究组孕妇胎盘组织中HBsAg及HBcAg的阳性率,呈现出由蜕膜细胞(76.3%及59.3%)、滋养层细胞(72.9%及55.9%)、绒毛间质细胞(62.7%及50.8%)至绒毛毛细血管内皮细胞(52.5%及44.1%)依次递减的趋势;但其中有4例孕妇胎盘组织中HBsAg及HBcAg的分布与上述特点相反.研究组孕妇有32例羊膜细胞中检出HBsAg及HBcAg.对照组孕妇胎盘组织中的HBsAg及HBcAg检出率为零.结论孕妇血中HBV主要是通过感染胎盘导致胎儿感染;但也可能存在胎盘以外的感染途径.
- [1]闫永平,徐德忠,王文亮.胎盘乙型肝炎病毒感染与宫内传播的关系[J].1999,(7).
- [2]王复生,李灼亮,张英,等.乙肝血清标记物阳性孕妇的胎盘组织和PBMC的HBVDNA检测及意义[J].1999,(1).
- [3]王珊珊,姜普林,彭桂福.乙型肝炎病毒的父儿传播与肝外定位[J].1999,(4).
- [4]Giovanna Lucifora,Santina Calabro,Giuseppe Carroccio,etc.Immunocytochemical HBsAg evidence in placentas of asymptomatic carrier mothers[J].1988,159(4).
- [5]Giovanna Lucifora,Fabio Martines,Santina Calabro,etc.\{HBcAg\} identification in the placental cytotypes of symptom-free HBsAg-carrier mothers: A study with the immunoperoxidase method[J].1990,163(Part 1).
- [6]周思亮,赵连三,黎飞,等.乙型肝炎患者卵细胞内检出乙肝病毒DNA[J].1989,(1).
- [7]Ho-Hsiung Lin,Tzu-Yao Lee,Ding-Shinn Chen,etc.Transplacental leakage ofHBeAg-positive maternal blood as the most likely route in causing intrauterine infection with hepatitis B virus[J].1987,111(Part 1).
- [8]Paul G. McDonough,P.J. Taylor,M.J. Gill,etc.Hepatitis B Virus in Human Follicular Fluid[J].1987,48(3).