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Study of the effects of utero-placental ischemia on the body and nervous system development in fetal rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 4
- DOI:
- 10.3760/j.issn:0529-567X.2004.04.002
- Key Word:
- 胎盘;缺血;GAP-43蛋白;胎儿发育
Abstract： 目的探讨不同孕龄孕鼠子宫、胎盘缺血对胎鼠体重及神经系统发育的影响.方法利用子宫动脉钳夹法对妊娠13 d(缺血1组)及妊娠17 d(缺血2组)孕鼠实施单侧子宫动脉缺血手术,制成孕鼠子宫动脉缺血模型.缺血组孕鼠的另一侧子宫动脉不钳夹为自身对照,相应为对照1组和对照2组.比较缺血组孕鼠子宫动脉钳夹缺血侧,与自身对照组孕鼠子宫动脉不钳夹侧(无缺血侧)的胎鼠体重、脑重及脑组织生长相关蛋白-43(GAP-43)mRNA的表达.结果 (1)缺血1组胎鼠体重、脑重分别为3.2 g和0.16 g,显著低于对照1组的3.6 g和0.18g(P＜0.01,P＜0.01);缺血2组的胎鼠体重、脑重分别为3.1g和0.16g,也显著低于对照2组的3.7g和0.17g(P＜0.01,P＜0.05).(2)缺血1组和对照1组胎鼠脑组织GAP-43mRNA的表达分别为1.10和1.12,两组比较,差异无显著性(P＞0.05);缺血2组与对照2组胎鼠脑组织GAP-43mRNA的表达分别为1.06和1.21,两组比较,差异有极显著性(P＜0.01).结论不同孕龄孕鼠子宫胎盘缺血均会显著抑制胎鼠的生长发育;妊娠晚期宫内缺血使胎脑组织GAP-43mRNA的表达显著减少,可对胎鼠的神经系统发育产生影响.
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