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Study on the association between maternal-infantile vertical transmission of hepatitis B virus and human leukocyte antigen DR gene domain

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Author:: No author available

Journal Title:: CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY

Issue:: 10

DOI:: 10.3760/j.issn:0529-567X.2003.10.003

Key Word:: 肝炎病毒,乙型;疾病传播,垂直;HLA-DR抗原;基因频率;基因型

Abstract：目的探讨人类白细胞抗原(HLA)DR区域基因HLA-DR3、HLA-DR4、HLA-DR13、HLA-DR15与乙型肝炎病毒(HBV)母婴垂直传播的关系.方法采用聚合酶链反应-序列特异性引物(PCR-SSP)方法,检测78例乙型肝炎表面抗原(HBsAg)阳性孕妇(研究组)和40例正常孕妇(对照组)外周静脉血中HLA-DR3、HLA-DR4、HLA-DR13、HLA-DR15基因表型分布及频率.结果 (1)研究组孕妇HLA-DR3基因频率为19.2%,显著高于对照组的5.0%(P<0.05);HLA-DR13基因频率为2.6%,显著低于对照组孕妇的17.5%(P<0.05);两组孕妇HLA-DR4 、HLA-DR15的基因频率比较,差异无显著性(P>0.05).(2)研究组孕妇中,HBV高复制状态者的HLA-DR3基因频率为30.0%,显著高于低复制状态者的7.9%(P<0.05).(3)将研究组孕妇分娩的新生儿,根据脐血清HBsAg、HBV DNA的检测结果分为:宫内感染组和宫内未感染组,宫内感染组孕妇HLA-DR3的基因频率为50.0%,显著高于宫内未感染组的14.7%(P<0.05).其余各基因表型比较,差异无显著性(P>0.05);宫内感染组新生儿HLA-DR3基因频率为30.0%,明显高于宫内未感染组的7.4%,但差异无显著性(P>0.05).结论孕妇HBsAg携带与HLA-DR3和HLA-DR13表达有明显相关性,其体内病毒高复制状态与HLA-DR3表达呈显著正相关;孕妇或胎儿携带HLA-DR3,易导致HBV母婴垂直传播.

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