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Expression of cyclooxygenase-2 mRNA and identification of its spliceriant in human myometrium obtained from women in labor
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 5
- DOI:
- 10.3760/j.issn:0529-567X.2003.05.005
- Key Word:
- 前列腺素内过氧化物合酶;DNA,互补;克隆,分子;序列分析;子宫肌层
Abstract： 目的寻找人子宫肌层诱导型环加氧酶 (cyclooxygenase-2,COX-2)选择性剪接变异体.方法运用自行设计的COX-2 mRNA引物,采用逆转录聚合酶链反应(RT-PCR)技术,分离临产与未临产产妇子宫肌层COX-2 mRNA,再对电泳所获条带进行亚克隆、测序.结果未临产产妇子宫肌层未检测到COX-2 mRNA,临产产妇子宫肌层不仅检测到COX-2 mRNA,而且分离出一新条带,经亚克隆及测序,证实该条带系人子宫肌层COX-2蛋白DNA的第7、8外显子间的内含子序列被保留所致.结论临产产妇子宫肌层COX-2 mRNA存在选择性剪接变异体.该变异体的发现,为分娩机制的阐明及前列腺素代谢异常相关性疾病的防治提供了新的研究方向.
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