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Tegument viral protein 22 enhanced cell-killing effect of the herpes simplex virus thymidine kinase/ganciclovir system on ovarian cancer in vivo
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 4
- DOI:
- 10.3760/j.issn:0529-567X.2003.04.002
- Key Word:
- 病毒结构蛋白质类;病毒壳体;胸苷激酶;更昔洛韦;卵巢肿瘤;肿瘤细胞,培养的;基因疗法
Abstract： 目的探讨病毒蛋白22(VP22)的细胞间传递作用,以及其促进单纯疱疹病毒胸苷激酶(HSV-TK )/更昔洛韦(GCV)基因治疗系统对卵巢癌移植瘤细胞的体内杀伤效应.方法用慢病毒感染卵巢癌3AO细胞,形成携带HSV-TK基因的3AO细胞(3AO/TK)及携带HSV-VP22-TK融合基因的3AO细胞(3AO/VP22-TK).分别在裸鼠皮下接种10%含卵巢癌3AO/TK(3AO/TK组)或3AO/VP22-TK(3AO/VP22-TK组)的混合细胞,并以接种单纯3AO细胞裸鼠为对照(3AO组),每组裸鼠10只.当肿瘤体积达150 mm3后,分别于腹腔注射GCV 10 mg/kg、50 mg/kg.结果注射10 mg/kg GCV后,3AO/TK组与3AO/VP22-TK组肿瘤体积、重量比较,差异有极显著性(P<0.01),3AO/VP22-TK组肿瘤生长明显受到抑制;注射50 mg/kg GCV后,两组肿瘤体积、重量比较,差异无显著性(P>0.05).注射GCV 10 mg/kg后,3AO/TK组肿瘤抑制率为37.7%,3AO/VP22-TK组肿瘤抑制率为91.5%,两组比较,差异有极显著性(P<0.01);注射GCV 50 mg/kg后,3AO/TK组肿瘤抑制率为81.8%,3AO/VP22-TK组肿瘤抑制率为96.7%,两组比较,差异无显著性(P>0.05).结论 VP22介导的自杀基因产物细胞间扩散作用,可明显加强对体内肿瘤细胞的杀伤效应.
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