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Study on prenatal diagnosis using fluorescence quantitative polymerase chain reaction for congenital toxoplasmosis
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 1
- DOI:
- 10.3760/j.issn:0529-567X.2003.01.003
- Key Word:
- 弓形虫病,先天性;产前诊断;聚合酶链反应
Abstract： 目的探讨荧光定量聚合酶链反应 (FQ-PCR ) 技术用于产前诊断胎儿弓形虫(TOX)感染及其防治措施.方法采用FQ-PCR技术,检测70例孕期TOX DNA阳性孕妇的羊水和(或)脐血的TOX DNA,阳性者为胎儿TOX感染.对其中48例TOX感染孕妇给予螺旋霉素常规剂量治疗2个疗程,观察其治疗效果.结果 (1)70例TOX DNA阳性孕妇中,胎儿TOX DNA阳性21例,胎儿宫内TOX感染率为30%,羊水和脐血中的TOX DNA 阴、阳性结果一致.(2)胎儿宫内TOX感染与孕妇血中TOX DNA含量高低有关,孕妇血中TOX DNA含量高时,对胎儿危害严重,胎儿宫内感染率也最高(55%);TOX DNA含量低时,胎儿宫内感染率也最低(20%),两者比较,差异有显著性(P<0.05).(3)经螺旋霉素治疗的孕妇,其胎儿宫内感染率为21%,明显低于未治疗者的50%, 两者比较,差异有显著性(P<0.05).结论孕妇TOX感染可危害胎儿,FQ-PCR检测羊水中TOX DNA可准确地诊断胎儿TOX感染,孕期治疗可降低宫内TOX感染率.
- [1]M H, Bessières,A, Berrebi,M, Rolland,etc.Neonatal screening for congenital toxoplasmosis in a cohort of 165 women infected during pregnancy and influence of in utero treatment on the results of neonatal tests.[J].2001,94.
- [2]Walter Foulon,Jean-Michel Pinon,Babill Stray-Pedersen,etc.Prenatal diagnosis of congenital toxoplasmosis: A multicenter evaluation of different diagnostic parameters[J].1999,181(4).
- [3]Villena I,Quereux C,Chemla C,etc.Prenatal diagnosis of congenital toxoplasmosis transmitted by an immunocompetent woman infected before conception. Reims Toxoplasmosis Group.[J].1998,18(10).
- [4]A, Berrebi,W E, Kobuch,M H, Bessieres,etc.Termination of pregnancy for maternal toxoplasmosis.[J].1994,344.