Study of relationship between T helper cell type-1 and type-2 cytokines and intrahepatic cholestasis of pregnancy
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 9
- DOI:
- 10.3760/j.issn:0529-567X.2002.09.002
- Key Word:
- 妊娠并发症;胆汁郁积,肝内;T淋巴细胞亚群;细胞活素类
Abstract： 目的探讨辅助性T淋巴细胞(TH)1型细胞因子[γ干扰素(IFN-γ)、肿瘤坏死因子α(TNF-α)]及TH2型细胞因子[白细胞介素4(IL-4)]与妊娠期肝内胆汁淤积症(ICP)发病的关系,从分子免疫学角度探讨ICP的发病机理.方法采用双抗体夹心酶联免疫吸附法,检测20例ICP孕妇(研究组)和20例正常妊娠妇女(对照组)外周血单个核细胞在植物血凝素刺激下产生TH1型细胞因子(IFN-γ,TNF-α)及TH2型细胞因子(IL-4)的水平,并对TH1型细胞因子与ICP患者血清总胆汁酸的相关性进行分析.结果 (1)研究组孕妇的IFN-γ、TNF-α水平分别为(639±156) ng/L、(1 021±231) ng/L;明显高于对照组孕妇的(464±147) ng/L、(728±175) ng/L,两组比较,差异有极显著性(P<0.001).研究组孕妇的IL-4水平为(22±4) ng/L,明显低于对照组孕妇的(27±6) ng/L,两组比较,差异有显著性(P<0.05).(2)研究组孕妇的TNF-α/IL-4比值为48±11, 明显高于对照组孕妇的30±8;研究组孕妇的IFN-γ/IL-4比值为30±8, 明显高于对照组的19±6,两组分别,差异均有极显著性(P<0.001).(3)研究组孕妇的IFN-γ、TNF-α水平与ICP患者血清胆汁酸水平呈正相关,相关系数(r)分别为0.458,0.692 (P<0.01).结论 ICP患者体内TH1/TH2型细胞因子平衡由TH2向TH1偏移,细胞免疫功能增强;ICP患者体内TH1型细胞因子免疫反应的增强可能与肝细胞损伤、肝内胆汁淤积症的发生有关.
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