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Anticancer effects of cytosine deaminase gene/5-fluorocytosine therapies to ovarian cancer in vivo
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 4
- DOI:
- 10.3760/j.issn:0529-567X.2002.04.002
- Key Word:
- 卵巢肿瘤;核苷酸脱氨酶类;氟胞嘧啶
Abstract： 目的了解胞嘧啶脱氨酶(CD)基因联合5-氟胞嘧啶(5-FC)对卵巢癌裸鼠皮下移植瘤生长的抑制作用.方法应用胚肾293细胞扩增整合有CD基因的复制缺陷的腺病毒,并将其分离、提纯.以卵巢癌裸鼠模型作为研究对象.实验组(5只):将整合有CD基因的复制缺陷的腺病毒0.1 ml[1×1010菌斑形成单位(pfu)/ml]注入瘤体内,隔日1次,共3次;同时将5-FC按每次400 mg/kg注入裸鼠腹腔内,每日2次,共7 d.对照组(5只):以同样方法将同体积生理盐水注入裸鼠体内.观察两组裸鼠的肿瘤生长速度及倍增时间.结果 (1)整合有CD基因的腺病毒滴度为1.07×1010 pfu/ml.(2)实验组裸鼠肿瘤生长明显受抑制,肿瘤体积由9 mm3增加到855 mm3,而对照组肿瘤体积由7 mm3增加到2 014 mm3,两组比较,差异有显著性( P <0.05),这种显著性差异在用药结束后约20 d才表现出来,并维持约30 d.(3)实验组与对照组肿瘤倍增时间分别为(8.1±0.7) d和(6.4±0.7) d,两组比较,差异有显著性( P <0.05).结论应用CD基因联合5-FC对卵巢癌裸鼠移植瘤具有良好的抑制作用.
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