Treatment of PC-3 cells with low-frequency and low-energy ultrasound combined with microbubbles induced distinct alterations in the expression of Bcl-2 and Bax
- Author:
- No author available
- Journal Title:
- Chinese Journal of Ultrasonography
- Issue:
- 10
- DOI:
- 10.3760/cma.j.issn.1004-4477.2012.10.024
- Key Word:
- 超声检查;微气泡;前列腺肿瘤;细胞凋亡;Ultrasonography;Microbubbles;Prostatic neoplasms;Apoptosis
Abstract： Objective To study whether low-frequency and low-energy ultrasound combined with microbubbles induced apoptotic cell death in androgen-independent prostate cancer cells and to identify the probable mechanism.Methods Ultrasound with a frequency of 21 kHz and intensity of 46 mW/cm2 in continuous wave mode was used.PC-3 cells were divided three groups:control group,ultrasound group and ultrasound combined with microbubbles.Ultrasound combined with microbubbles group was added 200 μl SonoVue.After treatment,colony assay was used to study cell proliferation.Twenty-four hours after treatment,transmission electron microscopy and TUNEL were used to measure cell apoptosis.Real-time PCR was used to measure the expression of the apoptosis-related mRNA,Bcl-2 and Bax.Results Ultrasound combined with microbubbles group's colony number was significantly less than the control group's colony number (153.33 ± 10.80 vs 313.33 ± 25.82,P ＜ 0.05).Ultrasound combined with microbubbles group induced more apoptosis in PC-3 cells than did the control group (13.33 ± 1.35 vs 0.33 ±0.17,P ＜0.05).Treatment with ultrasound combined with microbubbles decreased the expression of Bcl-2,an anti-apoptotic mRNA,and increased the expression of Bax,a pro-apoptotic mRNA.Conclusions Ultrasound combined with microbubbles induced apoptotic cell death in human prostate cancer PC-3 cells through down-regulation of Bcl-2 and up-regulation of Bax.
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