Sevoflurane postconditioning alleviates action potential duration shortening and L-type calcium current suppression induced by ischemia/reperfusion injury in rat epicardial myocytes

( views:155, downloads:0 )
Author:
GONG Jun-song(Department of Anesthesiology,Fuwai Hospital, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China)
YAO Yun-tai(Department of Anesthesiology,Fuwai Hospital, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China)
FANG Neng-xin(Department of Anesthesiology,Fuwai Hospital, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China)
HUANG Jian(Department of Pathology and Pathophysiology ,Fuwai Hospital, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China)
LI Li-huan(Department of Anesthesiology,Fuwai Hospital, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China)
Journal Title:
Chinese Medical Journal
Issue:
Volume 125, Issue 19, 2012
DOI:
10.3760/cma.j.issn.0366-6999.2012.19.022
Key Word:
sevoflurane; postconditioning; ischemia/reperfusion; L-type calcium current

Abstract: Background It has been proved that sevoflurane postconditioning (SpostC) could protect the heart against myocardial ischemia/reperfusion injury,however,there has been few research focused on the electrophysiological effects of SpostC.The objective of the study was to investigate the effects of SpostC on action potential duration (APD) and L-type calcium current (ICa,L) in isolated cardiomyocytes.Methods Langendorff perfused SD rat hearts were randomly assigned to one of the time control (TC),ischemia/reperfusion (I/R,25 minutes of ischemia followed by 30 minutes of reperfusion),and SpostC (postconditioned with 3% sevoflurane) groups.At the end of reperfusion,epicardial myocytes were dissociated enzymatically for patch clamp studies.Results Sevoflurane directly prolonged APD and decreased peak Ica,L densities in epicardial myocytes of the TC group (P<0.05).I/R injury shortened APD and decreased peak Ica,L densities in epicardial myocytes of the I/R group (P<0.05).SpostC prolonged APD and increased peak Ica,L densities in epicardial myocytes exposed to I/R injury (P<0.05).SpostC decreased intracellular reactive oxygen species (ROS) levels,reduced the incidence of ventricular tachycardia and ventricular fibrillation,and decreased reperfusion arrhythmia scores compared with the I/R group (all P<0.05).Conclusions SpostC attenuates APDshortening and ICa,L suppression induced by I/R injury.The regulation of APD and Ica,L by SpostC might be related with intracellular ROS modulation,which contributes to the alleviation of reperfusion ventricular arrhythmia.Chin Med J 2012;125(19):3485-3491

  • 1.Zhao ZQ,Vinten-Johansen J.Postconditioning:reduction of reperfusion-induced injury.Cardiovasc Res 2006; 70:200-211.
  • 2.Yao YT, Fang NX, Shi CX, Li LH.Sevoflurane postconditioning protects isolated rat hearts against ischemia-reperfusion injury.Chin Med J 2010; 123:1320-1328.
  • 3.Yao Y, Li L, Li L, Gao C, Shi C.Sevoflurane postconditioning protects chronically-infarcted rat hearts against ischemia-reperfusion injury by activation of pro-survival kinases and inhibition of mitochondrial permeability transition pore opening upon reperfusion.Biol Pharm Bull 2009; 32:1854-1861.
  • 4.Fang NX,Yao YT,Shi CX,Li LH.Attenuation of ischemia-reperfusion injury by sevoflurane postconditioning involves protein kinase B and glycogen synthase kinase 3 beta activation in isolated rat hearts.Mol Biol Rep 2010; 37:3763-3769.
  • 5.Yao YT,Li LH,Chen L,Wang WP,Li LB,Gao CQ.Sevoflurane postconditioning protects isolated rat hearts against ischemia-reperfusion injury:the role of radical oxygen species,extracellular signal-related kinases 1/2 and mitochondrial permeability transition pore.Mol Biol Rep 2010; 37:2439-2446.
  • 6.Obal D,Dettwiler S,Favoccia C,Scharbatke H,Preckel B,Schlack W.The influence of mitochondrial KATP-channels in the cardioprotection of preconditioning and postconditioning by sevoflurane in the rat in vivo.Anesth Analg 2005; 101:1252-1260.
  • 7.Walker M J,Curtis MJ,Hearse D J,Campbell RW,Janse M J,Yellon DM,et al.The Lambeth Conventions:guidelines for the study of arrhythmias in ischaemia infarction,and reperfusion.Cardiovasc Res 1988; 22:447-455.
  • 8.Curtis MJ,Walker MJ.Quantification of arrhythmias using scoring systems:an examination of seven scores in an in vivo model of regional myocardial ischaemia.Cardiovasc Res 1988; 22:656-665.
  • 9.Aizawa K,Turner LA,Weihrauch D,Bosnjak ZJ,Kwok WM.Protein kinase C-epsilon primes the cardiac sarcolemmal adenosine triphosphate-sensitive potassium channel to modulation by isoflurane.Anesthesiology 2004; 101:381-389.
  • 10.Michael G,Xiao L,Qi XY,Dobrev D,Nattel S.Remodelling of cardiac repolarization:how homeostatic responses can lead to arrhythmogenesis.Cardiovasc Res 2009; 81:491-499.
  • 11.Chae JE,Ahn DS,Kim MH,Lynch C 3rd,Park WK.Electrophysiologic mechanism underlying action potential prolongation by sevoflurane in rat ventricular myocytes.Anesthesiology 2007; 107:67-74.
  • 12.Rithalia A,Hopkins PM,Harrison SM.The effects of halothane,isoflurane,and sevoflurane on Ca2+ current and transient outward K+ current in subendocardial and subepicardial myocytes from the rat left ventricle.Anesth Analg 2004; 99:1615-1622.
  • 13.Qin D,Zhang ZH,Caref EB,Boutjdir M,Jain P,el-Sherif N.Cellular and ionic basis of arrhythmias in postinfarction remodeled ventricular myocardium.Circ Res 1996; 79:461-473.
  • 14.Yildirim H,Adanir T,Atay A,Katircio(g)lu K,Savaci S.The effects of sevoflurane,isoflurane and desflurane on QT interval of the ECG.Eur J Anaesthesiol 2004; 21:566-570.
  • 15.Drew BJ,Ackerman MJ,Funk M,Gibler WB,Kligfield P,Menon V,et al.Prevention of torsade de pointes in hospital settings:a scientific statement from the American Heart Association and the American College of Cardiology Foundation.Circulation 2010; 121:1047-1060.
  • 16.Coetzee WA,Opie LH.Effects of oxygen free radicals on isolated cardiac myocytes from guinea-pig ventricle:electrophysiological studies.J Mol Cell Cardiol 1992; 24:651-663.
  • 17.Goldhaber JI,Ji S,Lamp ST,Weiss JN.Effects of exogenous free radicals on electromechanical function and metabolism in isolated rabbit and guinea pig ventricle.Implications for ischemia and reperfusion injury.J Clin Invest 1989; 83:1800-1809.
  • 18.Bodi I,Mikala G,Koch SE,Akhter SA,Schwartz A.The L-type calcium channel in the heart:the beat goes on.J Clin Invest 2005; 115:3306-3317.
  • 19.Chen Q,Camara AK,An J,Novalija E,Riess ML,Stowe DE Sevoflurane preconditioning before moderate hypothermic ischemia protects against cytosolic[Ca(2+)]loading and myocardial damage in part via mitochondrial K(ATP)channels.Anesthesiology 2002; 97:912-920.
  • 20.González G,Zaldívar D,Carrillo E,Hemández A,García M,Sánchez J.Pharmacological preconditioning by diazoxide downregulates cardiac L-type Ca(2+) channels.Br J Pharmacol 2010; 161:1172-1185.
  • 21.Koval OM,Guan X,Wu Y,Joiner ML,Gao Z,Chen B,et al.CaV1.2 beta-subunit coordinates CaMKⅡ-triggered cardiomyocyte death and afterdepolarizations.Proc Natl Acad Sci U S A 2010; 107:4996-5000.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn