Increased expression of stathmin in eutopic endometrium of patients with endometriosis

( views:53, downloads:0 )
LI Chun-yan(Department of Obstetrics and Gynecology, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, China)
LIU Hai-yuan(Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China)
LANG Jing-he(Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China)
WANG Hong-qing(Department of Obstetrics and Gynecology, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, China)
FAN Xiu-ling(Department of Obstetrics and Gynecology, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, China)
Journal Title:
Volume 123, Issue 16, 2010
Key Word:
stathmin;endometriosis;real-time PCR;eutopic endometrium

Abstract: Background Stathmin was identified as an endometriosis-related protein by comparative proteomics in our previous study.As a microtubule-destablizing factor, stathmin was shown to participate in the relay and integration of diverse intracellular signaling pathways involved in cell proliferation, differentiation, and many other cellular activities.To investigate whether stathmin is involved in the pathogenesis of endometriosis, we examined the expression of stathmin in eutopic endometrium of women with or without endometriosis.Methods Eutopic endometrium samples were collected from thirty-six patients who were diagnosed as endometriosis and the nineteen age-matched patients who were confirmed to be free of endometriosis surgically and histologically.The expression of stathmin mRNA was detected by real-time PCR, and its protein was detected by Western blotting and immunohistochemistry.Results Stathmin was overexpressed in eutopic endometrium of women with endometriosis detected by real-time PCR in mRNA levels and by Western blotting in protein levels, without significant difference between proliferative and 0secretory phase.Immunohistochemistry showed that stathmin protein was localized in both endometrial glandular and stromal cells throughout the menstrual cycle.Conclusions Stathmin is overexpressed in endometrium of patients with endometriosis and may play a role in the pathogenesis of endometriosis.

  • [1]Bartosik D,Jacobs SL,Kelly LJ.Endometrial tissue in peritoneal fluid.Fertil Steril 1986; 46:796-800.
  • [2]Oral E,Arici A.Pathogenesis of endometriosis.Obstet Gynecol Clin North Am 1997; 24:219-233.
  • [3]Goldman MB,Cramer DW.The epidemiology of endometriosis.Prog Clin Biol Res 1990; 323:15-31.
  • [4]Ulukus M,Cakmak H,Arici A.The role of endometrium in endometriosis.J Soc Gynecol Investig 2006; 13:467-476.
  • [5]Tan X J,Lang JH,Liu DY,Shen K,Leng JH,Zhu L.Expression of vascular endothelial growth factor and thrombospondin-1 mRNA in patients with endometriosis.Fertil Steril 2002; 78:148-153.
  • [6]Liu H,Lang J,Wang X,Wu S.Comparative proteomic analysis of human adenomyosis using two-dimensional gel electrophoresis and mass spectrometry.Fertil Steril 2008; 89:1625-1631.
  • [7]Rubin CI,Atweh GF.The role of stathmin in the regulation of the cell cycle.J Cell Biochem 2004; 93:242-250.
  • [8]Sobel A,Boutterin MC,Beretta L,Chneiweiss H,Doye V,Peyro-Saint-Paul H.Intracellular substrates for extracellular signaling.Characterization of a ubiquitous,neuron-enriched phosphoprotein (stathmin).J Biol Chem 1989; 264:3765-3772.
  • [9]Marklund U,Larsson N,Gradin HM,Brattsand G,Gullberg M.Oncoprotein 18 is a phosphorylation-responsive regulator of microtubule dynamics.EMBO J 1996; 5:5290-5298.
  • [10]Alli E,Yang JM,Hait WN.Silencing of stathmin induces tumor-suppressor function in breast cancer cell lines harboring mutant p53.Oncogene 2007; 26:1003-1012.
  • [11]Mistry SJ,Bank A,Atweh GF.Targeting stathmin in prostate cancer.Mol Cancer Ther 2005; 4:1821-1829.
  • [12]Burlev VA,Pavlovich SV,Il'yasova NA.Apoptosis and proliferative activity in endometrium during peritoneal endometriosis.Bull Exp Biol Med 2006; 141:204-207.
  • [13]Béliard A,No(e)l A,Foidart JM.Reduction of apoptosis and proliferation in endometriosis.Fertil Steril 2004; 82:80-85.
  • [14]Hanash SM,Strahler JR,Kuick R,Chu EH,Nichols D.Identification of a polypeptide associated with the malignant phenotype in acute leukemia.J Biol Chem 1988; 263:12813-12815.
  • [15]Luo XN,Arcasoy MO,Brickner HE,Mistry S,Schechter AD,Atweh GF.Regulated expression of p18,a major phosphoprotein of leukemic cells.J Biol Chem 1991; 266:21004-21010.
  • [16]Jeha S,Luo XN,Beran M,Kantarjian H,Atweh GF.Antisense RNA inhibition of phosphoprotein p18 expression abrogates the transformed phenotype of leukemic cells.Cancer Res 1996;56:1445-1450.
  • [17]Iancu C,Mistry S J,Arkin S,Wallenstein S,Atweh GF.Effects of stathmin inhibition on the mitotic spindle.J Cell Sci 2001;114:909-916.
  • [18]Price DK,Ball JR,Bahrani-Mostafavi Z,Vachris JC,Kaufman JS,Naumann RW,et al.The phosphoprotein Op18/stathmin is differentially expressed in ovarian cancer.Cancer Invest 2000;18:722-730.
  • [19]Balogh A,Mège RM,Sobel A.Growth and cell density-dependent expression of stathmin in C2 myoblasts in culture.Exp Cell Res 1996; 224:8-15.
  • [20]Rowlands DC,Harrison RF,Jones NA,Williams A,Hubscher SG,Brown G.Stathmin is expressed by the proliferating hepatocytes during liver regeneration.Clin Mol Pathol 1995;48:88-92.
  • [21]Zahedi K,Wang Z,Barone S,Tehrani K,Yokota N,Petrovic S,et al.Identification of stathmin as a novel marker of cell proliferation in the recovery phase of acute ischemic renal failure.Am J Physiol Cell Physiol 2004; 286:C1203-C1211.
  • [22]Tamura K,Yoshie M,Nishi H,Osakabe Y,lsaka K,Hara T,et al.Expression of stathmin in human uterus and decidualizing endometrial stromal cells.Reproduction 2006; 132:625-636.
  • [23]Yoshie M,Tamura K,Hara T,Kogo H.Expression of stathmin family genes in the murine uterus during early pregnancy.Mol Reprod Dev 2006; 73:164-172.
  • [24]Tamura K,Yoshie M,Hara T,Isaka K,Kogo H.Involvement of stathmin in proliferation and differentiation of immortalized human endometrial stromal cells.J Reprod Dev 2007; 53:525-533.
  • [25]Becker CM,Bartley J,Mechsner S,Ebert AD:Angiogenesis and endometriosis.Zentralbl Gynakol 2004; 126:252-258.
  • [26]Hur SE,Lee JY,Moon HS,Chung HW.Angiopoietin-1,angiopoietin-2 and Tie-2 expression in eutopic endometrium in advanced endometriosis.Mol Hum Reprod 2006; 12:421-426.
  • [27]Dabrosin C,Gyorffy S,Margetts P,Ross C,Gauldie J.Therapeutic effect of angiostatin gene transfer in a murine model of endometriosis.Am J Pathol 2002; 161:909-918.
  • [28]Miyashita H,Kanemura M,Yamazaki T,Abe M,Sato Y.Vascular endothelial zinc finger I is involved in the regulation of angiogenesis:possible contribution of stathmin/OP18 as a downstream target gene.Arterioscler Thromb Vasc Biol 2004;24:878-884.
  • [29]Mistry SJ,Bank A,Atweh GF.Synergistic antiangiogenic effects of stathmin inhibition and taxol exposure.Mol Cancer Res 2007; 5:773-782.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615