Changes of cathepsin B in human photoaging skin both in vivo and in vitro
- Author:
- No author available
- Journal Title:
- CHINESE MEDICAL JOURNAL
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.0366-6999.2010.05.004
- Key Word:
- cathepsin B;ultraviolet light;photoaging;skin
Abstract： Background Cathepsin B plays an important role in cell cycle, extracellular matrix changes and cutaneous tumorigenesis: whether it plays a role in photoaged skin remains unknown. This study aimed to investigate the role of cathepsin B in skin photoaging in vivo and in vitro. Methods The expressions of cathepsin B were compared with immunohistochemical methods in solar exposed skin and solar protected skin of six healthy Chinese volunteers. The mRNA and protein expression of cathepsin B in ultraviolet light A (UVA) induced premature senescence fibroblasts in vitro were detected by real-time reverse transcription polymerase chain reaction (RT-PCR) and Western blotting technique. Results Decreased expression of cathepsin B was observed in photoaged skin compared with that of the solar protected skin. In the UVA induced, premature senescence fibroblasts, a lower expression of cathepsin B was detected by Western blotting and a decreased synthesis of cathepsin B mRNA in the same cells was revealed by real-time RT-PCR. Conclusions The results demonstrated a significant negative correlation between skin photoaging and cathepsin B in vitro and in vivo. We propose that cathepsin B, besides matrix metalloproteinases and antioxidant enzymes, is involved in the process of skin photoaging in that it contributes to extracellular matrix remodelling and is a dominant protease in cellular apoptosis and senescence.
- [1]WANG XIao-yong,BI Zhi-gang.UVB-irradiated human keratinocytes and interleukin-1αindirectly increase MAP kinase/AP-1 activation and MMP-1 production in UVA-irradiated dermal fibroblasts[J].2006,(10).
- [2]Ma W,Jansen Durr P,Wlaschek M,etc.Photoaging as a consequence of natural and therapeutic ultraviolet irradiation-studies on PUVA-induced senescence-like growth arrest of human dermal fibroblasts.[J].2003,38(11).
- [3]D, Gabrijelcic,R, Gollwitzer,T, Popovi,etc.Proteolytic cleavage of human fibrinogen by cathepsin B.[J].1988,369 Suppl.
- [4]T T, Lah,M R, Buck,K V, Honn,etc.Degradation of laminin by human tumor cathepsin B.[J].1900,7.
- [5]Wlaschek M,Picardo M,Scharffetter-Kochanek K,etc.Small molecular antioxidants effectively protect from PUVA-induced oxidative stress responses underlying fibroblast senescence and photoaging.[J].2008,45(5).
- [6]Hinrichs C,Debacq-Chainiaux F,Wlaschek M,etc.The gene expression profile of psoralen plus UVA-induced premature senescence in skin fibroblasts resembles a combined DNA-damage and stress-induced cellular senescence response phenotype.[J].2007,42(9).
- [7]B F, Sloane,J R, Dunn,K V, Honn.Lysosomal cathepsin B: correlation with metastatic potential.[J].1981,212.
- [8]Rhie G,Shin MH,Seo JY,etc.Aging- and photoaging-dependent changes of enzymic and nonenzymic antioxidants in the epidermis and dermis of human skin in vivo.[J].2001,117(5).
- [9]Brix K,Ostafe R,Dannenmann SR,etc.Cathepsin B is essential for regeneration of scratch-wounded normal human epidermal keratinocytes[J].2007,86(11).
- [10]L.B. Creemers,K.A. Hoeben,D.C. Jansen,etc.Participation of intracellular cysteine proteinases, in particular cathepsin B, in degradation of collagen in periosteal tissue explants[J].1998,16(9).
- [11]A. KAWADA,K. HARA,E. KOMINAMI,etc.Expression of cathepsin D and B in invasion and metastasis of squamous cell carcinoma[J].1997,137(3).
- [12]Caballero B,De-Gonzalo-Calvo D,Camins A,etc.Melatonin alters cell death processes in response to age-related oxidative stress in the brain of senescence-accelerated mice.[J].2009,46(1).
- [13]Eiki Kominami,Kazumi Ishidoh.Gene Regulation and Extracellular Functions of Procathepsin L[J].1998,379(2).
- [14]Mansoureh Sameni,Tom Mikkelsen,Bonnie F. Sloane,etc.Degradation of Extracellular Matrix Protein Tenascin-C by Cathepsin B:An Interaction Involved in the Progression of Gliomas[J].2002,383(9).
- [15]Sang Yun Cho,Soo-Han Bae,Young-Ki Paik,etc.Differential expression of the liver proteome in senescence accelerated mice[J].2003,3(10).
- [16]Chen BX,Yu Y,Guo Q,etc.Cathepsin B and its interacting proteins, bikunin and TSRC1, correlate with TNF-induced apoptosis of ovarian cancer cells OV-90[J].2006,580(1).
- [17]Nusgens B.,Lambert C.,Colige A.,etc.Changes in matrix gene and protein expressions after single or repeated exposure to one minimal erythemal dose of solar-simulated radiation in human skin in vivo[J].2004,79(3).
- [18]L.Declercq,I.Sente,L.Hellemans,etc.Use of the synthetic superoxide dismutase/catalase mimetic EUK-134 to compensate for seasonal antioxidant deficiency by reducing pre-existing lipid peroxides at the human skin surface[J].2004,26(5).
- [19]Frohlich E,Mohrle M,Klessen C.Cathepsins in basal cell carcinomas: activity, immunoreactivity and mRNA staining of cathepsins B, D, H and L.[J].2004,295(10).