Endothelial cell chimerism by fluorescence in situ hybridization in gender mismatched renal allograft biopsies
- Author:
- No author available
- Journal Title:
- CHINESE MEDICAL JOURNAL
- Issue:
- 10
- DOI:
- No doi available
- Key Word:
- kidney transplantation;in situ hybridization,fluorescence;endothelium,vascular;chimerism;cold ischemia
Abstract： Background The blood vessels of a transplanted organ are the interface between donor and recipient. The endothelium in the blood vessels is thought to be the major target for graft rejection. Endothelial cells of a transplanted organ can be of recipient origin after transplantation. In this study, we tested whether endothelial chimerism correlated with the graft rejection and cold ischemia.Methods We studied the biopsy samples from 34 renal transplants of female recipients who received the kidney from a male donor for the presence of endothelial cells of recipient origin. We examined the tissue sections of renal biopsy samples by fluorescence in situ hybridization (FISH) for the presence of endothelial cells containing two X chromosomes using a biotinylated Y chromosome probe and digoxigenin labelled X chromosome probe, and then analyzed the relationship between the endothelial cell chimerism and the rejection and cold ischemia.Results Endothelial chimerism was common and irrespective of rejections (P＞0.05). The cold ischemic time of chimerism group was longer than no chimerism group ((14.83±4.03) hours vs (11.27±3.87) hours, P＜0.05).Conclusions There is no correlation between the percentage of recipient endothelial cells in vascular endothelial cells and the type of graft rejection. The endothelium damaged by ischemic injury might be repaired by the endothelial cells from the recipient.
- [1]R A, Sinclair.Origin of endothelium in human renal allografts.[J].1972,4(5831).
- [2]Parmjeet S. Randhawa,Thomas Starzl,Hector C. Ramos,etc.Allografts Surviving for 26 to 29 Years Following Living-Related Kidney Transplantation: Analysis by Light Microscopy, In Situ Hybridization for the Y Chromosome, and Anti-HLA Antibodies[J].1994,24(1).
- [3]Salazar AB,McAlister VC,MacDonald AS,etc.Circulating endothelial cells after transplantation.[J].2001,357(9266).
- [4]J F, Burdick,P S, Russell,H J, Winn.Sensitivity of long-standing xenografts of rat hearts to humoral antibodies.[J].1979,123(4).
- [5]M. L. Rose.Endothelial cells as antigen-presenting cells: role in human transplant rejection[J].1998,54(9).
- [6]R H, Hruban,P P, Long,E J, Perlman,etc.Fluorescence in situ hybridization for the Y-chromosome can be used to detect cells of recipient origin in allografted hearts following cardiac transplantation.[J].1993,142.
- [7]S, Rafii,F, Shapiro,J, Rimarachin,etc.Isolation and characterization of human bone marrow microvascular endothelial cells: hematopoietic progenitor cell adhesion.[J].1994,84.
- [8]Navaratnarasah S,Hodivala-Dilke K,Wright NA,etc.Bone marrow contributes to renal parenchymal turnover and regeneration.[J].2001,195(2).
- [9]van-Krieken JH,van-Kemenade FJ,Lagaaij EL,etc.Endothelial cell chimerism after renal transplantation and vascular rejection.[J].2001,357(9249).
- [10]G M, Williams,A, ter Haar,L C, Parks,etc.Endothelial changes associated with hyperacute, acute, and chronic renal allograft rejection in man.[J].1973,5.
- [11]Eveline P, van Poelgeest,Hans J, Baelde,Emma L, Lagaaij,etc.Endothelial cell chimerism occurs more often and earlier in female than in male recipients of kidney transplants.[J].2005,68(2).
- [12]Pons JA,Yelamos J,Robles R,etc.Endothelial cell chimerism does not influence allograft tolerance in liver transplant patients after withdrawal of immunosuppression.[J].2003,75(7).
- [13]C B, Andersen,S D, Ladefoged,S, Larsen.Cellular inflammatory infiltrates and renal cell turnover in kidney allografts: a study using in situ hybridization and combined in situ hybridization and immunohistochemistry with a Y-chromosome-specific DNA probe and monoclonal antibodies.[J].1991,99.
- [14]Solez K,Hill CM,Gibson IW,etc.The Banff 97 working classification of renal allograft pathology.[J].1999,55(2).
- [15]Tolkoff Rubin NE,Shih VE,Picard MH,etc.Vascular endothelial function in cyclosporine and tacrolimus treated renal transplant recipients.[J].2001,72(8).
- [16]P. B.Medawar.Transplantation of Tissues and Organs: Introduction[J].1965,21(2).
- [17]M J, Bogman,R M, de Waal,R A, Koene.Persistent expression of donor antigens in endothelium of long-standing skin xenografts and vulnerability to destruction by specific antibodies.[J].1987,19.
- [18]Antoni, Bayes-Genis,Marta, Salido,Francesc, Solé Ristol,etc.Host cell-derived cardiomyocytes in sex-mismatch cardiac allografts.[J].2002,56(3).