Effects of TNF-α and curcumin on the expression of VEGF in Raji and U937 cells and on angiogenesis in ECV304 cells
- Author:
- No author available
- Journal Title:
- CHINESE MEDICAL JOURNAL
- Issue:
- 24
- DOI:
- No doi available
- Key Word:
- tumour necrosis factors-α;curcumin;vascular endothelial growth factors;Notch1
Abstract： Background To better understand the possibilities of antiangiogenic tumor therapy and to assess possible side effects, we investigated the effect of tumour necrosis factor (TNF)-α and curcumin on the expression of vascular endothelial growth factor (VEGF) in U937 and Raji cell lines and their effect on angiogenesis in a human umbilical vein endothelial cell (HUVECs)-derived cell line (ECV304), and also the relationship between Notch1 and VEGF. The aim of this study was to elucidate potential mechanisms controlling tumor neovascularization. Methods VEGF secreted by U937 and Raji cell lines was determined by ELISA. Angiogenesis was tested by network formation of endothelial cells on Matrigel. Levels of VEGF mRNA in U937 and Raji cells and Notch1 mRNA levels in EV304 cells were determined by RT-PCR. Results Secretion of VEGF by U937 and Raji cells was increased by TNF-α treatment and suppressed by curcumin (P<0.01). The mRNA expression of VEGF165 and VEGF121 (containing 165 and 121 amino acid residues, respectively) were detected in any fractions. TNF-α augmented the expression of VEGF165 and VEGF121 mRNA and curcumin reduced the expression (P<0.01). No networks or cords formed in control and curcumin groups. There was tube formation on matrigel in the supernatants of the Raji culture group and the supernatants groups treated by VEGF group and TNF-α in Raji cell. Notch1 mRNA was detected but there was no significant change in the VEGF group compared with control (P>0.05).Conclusions Expressions of VEGF mRNA in U937 and Raji cells were increased by TNF-α and suppressed by curcumin. VEGF and TNF-α can induce angiogenesis, and curcumin can inhibit angiogenesis in ECV304 cells.
- [1]A. Vacca,D. Ribatti,M. Iurlaro,etc.Human lymphoblastoid cells produce extracellular matrix-degrading enzymes and induce endothelial cell proliferation, migration, morphogenesis, and angiogenesis[J].1998,28(1).
- [2]Alitalo K,Karpanen T,Saaristo A.Mechanisms of angiogenesis and their use in the inhibition of tumor growth and metastasis.[J].2000,19(53).
- [3]Y, Kubota,H K, Kleinman,G R, Martin,etc.Role of laminin and basement membrane in the morphological differentiation of human endothelial cells into capillary-like structures.[J].1988,107.
- [4]Kui Huang,Charlotte Andersson,Godfried M. Roomans,etc.Signaling properties of VEGF receptor-1 and -2 homo- and heterodimers[J].2001,33(4).
- [5]Sainson RC,Aoto JN,Aitkenhead M,etc.Angiogenic sprouting and capillary lumen formation modeled by human umbilical vein endothelial cells (HUVEC) in fibrin gels: the role of fibroblasts and Angiopoietin-1 small star, filled.[J].2003,66(2).
- [6]Kotani K.,Ogawa W.,Hino Y.,etc.Dominant Negative Forms of Akt (Protein Kinase B) and Atypical Protein Kinase C\u00a0 Do Not Prevent Insulin Inhibition of Phosphoenolpyruvate Carboxykinase Gene Transcription[J].1999.
- [7]Liu ZJ,Mine S,Ueda T,etc.A critical role for cyclin C in promotion of the hematopoietic cell cycle by cooperation with c-Myc.[J].1998,18(6).
- [8]Wang SJ,Hughes CC,Aitkenhead M,etc.The basic helix-loop-helix transcription factor HESR1 regulates endothelial cell tube formation.[J].2001,276(9).
- [9]Nishio I,Hano T,Imanishi T.Angiotensin II potentiates vascular endothelial growth factor-induced proliferation and network formation of endothelial progenitor cells.[J].2004,27(2).
- [10]J A, Forsythe,B H, Jiang,N V, Iyer,etc.Activation of vascular endothelial growth factor gene transcription by hypoxia-inducible factor 1.[J].1996,16.
- [11]Weinmaster G,Gao X,Chang B,etc.Embryonic lethality and vascular defects in mice lacking the Notch ligand Jagged1.[J].1999,8(5).
- [12]Houck Keith A.,Ferrara Napoleone,Winer Jane,etc.The Vascular Endothelial Growth Factor Family: Identification of a Fourth Molecular Species and Characterization of Alternative Splicing of RNA[J].1991.
- [13]A E, Kossakowska,A, Hinek,D R, Edwards,etc.Proteolytic activity of human non-Hodgkin's lymphomas.[J].1998,152.
- [14]Li Y,Soma A,Oka M,etc.Regulation of Notch1 and Dll4 by vascular endothelial growth factor in arterial endothelial cells: Implications for modulating arteriogenesis and angiogenesis[J].2003,23(1).