Abstract： Background Prehospital delay remains one of the main causes of reduced benefit of reperfusion therapy for patients with acute myocardial infarction (AMI). The largest proportion of prehospital delay involves the interval between the onset of symptoms and the decision to seek medical treatment. The purpose of This study was to examine the factors associated with the extent of care-seeking delay in Beijing for patients with AMI. Methods A structured interview was conducted in 102 patients with AMI in eight hospitals in Beijing.Results The mean decision time in patients with AMI was (204±43) minutes, and prehospital delay time was (311±54) minutes. Only 34% of patients sought medical care within one hour and a further 36% of patients presented to one of the eight hospitals within two hours after onset. Educational level, atypical presentation of AMI, and family members at the site where AMI occurred were associated with longer delay time in seeking medical assistance (P<0.05, respectively), whereas the intensity of chest pain was inversely related to patients' delay time (P<0.01). Patients who perceived their family relationship as good, attributed their symptoms to AMI origin, knew the time-dependent nature of reperfusion therapy, or used emergency medical service tended to seek medical care in a more rapid manner (P<0.05, respectively).Conclusions Patients with AMI in Beijing delay seeking medical care to a great extent. Health education to increase the level of awareness of the target population at increased risk of AMI, including patients and their family members, is probably beneficial to reduce patients' care-seeking delay.