Insulin-like growth factor binding protein 1 and human embryonic development during 6-10 gestational weeks
- Author:
- No author available
- Journal Title:
- CHINESE MEDICAL JOURNAL
- Issue:
- 4
- DOI:
- No doi available
- Key Word:
- insulin-like growth-factor binding protein 1;embryology;pregnancy
Abstract： Background Insulin-like growth factor binding protein-1 (IGFBP-1), which is a carrier of Insulin-like growth factors (IGFs) regulates the fetal development by working as an active factor controlling the combination of IGFs with their receptors. This study was designed to investigate the relationship between IGFBP-1 and human embryonic development during weeks 6 -10 of gestation.Methods A total of 44 pregnant women with singleton pregnancy were divided into two groups: one with abnormal embryo development (n = 32) and the other with normal embryo development (n = 12).Enzyme-linked immunosorbent assay (ELISA) was employed to detect IGFBP-1 levels in maternal serum and decidual tissue. The expression of IGFBP-1 mRNA in deciduas was examined by reverse transcription polymerase chain reaction (RT-PCR) technique.Results The level of IGFBP-1 protein in maternal serum was significantly higher in the abnormal group [ (125.36 ± 47.93) μg/ml] than in the normal group [(70.72 ± 21.21) μg/ml ]. Both of IGFBP-1 and IGFBP-1 mRNA in deciduas were higher in abnormal group [ (1.60 ± 1.39) μg/ml and 1.66 ± 1.64, respectively ] than in the normal group [ (0.35 ± 0.23) μg/mi and 0.40 ± 0.20,respectively]. The level of IGFBP-1 in maternal serum was positively correlated with IGFBP-1 mRNA (r=0. 90, P＜0.05) and IGFBP-1 protein (r=0.92, P＜0.05) in decidual tissue.Conclusions During weeks 6 -10 of gestation, abnormal embryonic development is correlated with elevated IGFBP-1. The level of IGFBP-1 in maternal serum is related to the concentrations of IGFBP1 mRNA and IGFBP-1 in decidual tissue. The IGFBP-1 level in maternal serum may be used as a predictive marker to evaluate embryonic development.
- [1]L J, Murphy,K, Rajkumar,P, Molnar.Phenotypic manifestations of insulin-like growth factor binding protein-1 (IGFBP-1) and IGFBP-3 overexpression in transgenic mice.[J].1995,6(2-4).
- [2]L C, Giudice,N A, Martina,R A, Crystal,etc.Insulin-like growth factor binding protein-1 at the maternal-fetal interface and insulin-like growth factor-I, insulin-like growth factor-II, and insulin-like growth factor binding protein-1 in the circulation of women with severe preeclampsia.[J].1997,176(4).
- [3]Ranke M.B.,Elmlinger M..Functional Role of Insulin-Like Growth Factor Binding Proteins[J].1997,48(Suppl 4).
- [4]L. J. Murphy.Overexpression of insulin-like growth factor binding protein-1 in transgenic mice[J].2000,14(7).
- [5]J I, Jones,D R, Clemmons.Insulin-like growth factors and their binding proteins: biological actions.[J].1995,16.
- [6]Giudice L C,de Zegher F,Gargosky S E,etc.Insulin-like growth factors and their binding proteins in the term and preterm human fetus and neonate with normal and extremes of intrauterine growth.[J].1995.
- [7]Han VK,Carter AM.Spatial and temporal patterns of expression of messenger RNA for insulin-like growth factors and their binding proteins in the placenta of man and laboratory animals.[J].2000,21(4).
- [8]Irwin JC,Giudice LC,Carland G,etc.Hypoxia stimulates insulin-like growth factor binding protein 1 (IGFBP-1) gene expression in HepG2 cells: a possible model for IGFBP-1 expression in fetal hypoxia.[J].1998,95(17).
- [9]Wang H. S.,Chard T..The role of insulin-like growth factor-I and insulin-like growth factor-binding protein-1 in the control of human fetal growth[J].1992.
- [10]J C, Irwin,L F, Suen,N A, Martina,etc.Role of the IGF system in trophoblast invasion and pre-eclampsia.[J].1999,14.