Cloning the full-length cDNA of a gene responsible for vascular smooth muscle cell proliferation in atherogenesis and study of function
- Author:
- No author available
- Journal Title:
- CHINESE MEDICAL JOURNAL
- Issue:
- 2
- DOI:
- No doi available
- Key Word:
- subtractive hybridization;associated gene;cDNA;whole length;functional study
Abstract： Objective To clone the full-length cDNA of a gene responsible for vascular smooth muscle cell (v-SMC) proliferation in atherogenesis, and study its function.Methods Oxidized low density lipoprotein (ox-LDL) at optimal concentration was used as the stimulant to induce v-SMC prol iferation in culture medium. AcDNA subtractive library of v-SMC proliferation specific toox-LDL stimulation was established using subtractive hybridization technique. Methods, including blotting, Northern hybridization and gene sequen cing, were used to clone new gene fragments. By using full-length cDNA screeni ng and protein expression techniques, one full-length cDNA was cloned and its f unction was studied. Results One full-length cDNA was cloned. The new gene (Genbank AF 174647) expressed a 44 kDa protein, which might be associated with the activity of ox-LDL. Conclusion The new gene cloned may be associated with SMC proliferation in atherogenesis.
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